Charlotte M. Miton

This study maps substrate-dependent epistasis in phosphotriesterase by analyzing 64 mutation combinations across nine substrates, revealing how intramolecular wiring changes to support promiscuity and functional trade-offs.

This paper introduces LASE, a method using ancestral sequence reconstruction to train protein language models with higher accuracy and faster computation than large models.

The paper describes how two distinct enzymes evolved from a common ancestor through different mutational paths, leading to divergent molecular and phenotypic outcomes.