Emily M. Pujadas-Liwag

This study shows that HP1 stabilizes chromatin packing domains by maintaining their structure and enabling efficient transcriptional responses to stress.

The paper shows that Pol-II regulates chromatin packing domains through transcriptional loops, linking RNA synthesis to genome organization and gene expression.

The paper investigates how nuclear blebs alter chromatin packing domains, finding fragmentation of nanoscopic heterochromatin domains within blebs.

This paper introduces csPWS-seg, a deep learning model for automated nuclei segmentation in label-free live cell csPWS microscopy, achieving high accuracy with median IoU 0.80 and DSC 0.88.

The paper compares lamin B and DNA levels in nuclear blebs across cell types and perturbations, finding DNA concentration is more consistently reduced than lamin B levels.

The paper shows that RNA polymerase I co-regulates chromatin domains and mRNA synthesis with RNA polymerase II, affecting genome connectivity and gene expression.

This study shows that formamide in 3D FISH distorts nanoscale chromatin structure, while alternative methods like RASER-FISH and CRISPR-Sirius have minimal impact.

The paper shows that RAD21 depletion reduces but does not eliminate chromatin packing domains in electron microscopy, suggesting non-cohesin mechanisms largely control 3D structure.

The paper introduces a nano-ChIA platform that combines electron tomography and superresolution imaging to study chromatin structure and dynamics at the single-nucleosome level in live cells.