Jason L. Rasgon

The paper introduces GEValT, a method for heritable CRISPR gene editing in arthropods via oral viral transduction, enabling efficient gene knock-out and knock-in.

This study uses small RNA-seq to analyze the virome of Culex tarsalis mosquitoes despite suboptimal sample conditions, identifying seven viruses and their small RNA profiles.

This study examines the hologenomic structure of bacterial and fungal communities in Culex tarsalis mosquitoes, finding geographic and environmental influences on microbiome composition and partial correlation with host genetics.

This study uses cryo-electron tomography to reveal the nanoscale architecture of Wolbachia pipientis structures involved in host manipulation and maternal inheritance.

This paper develops an AgDNV intronic expression system to deliver microRNAs in Anopheles gambiae, showing variable miRNA target gene regulation.

This study examines how prolonged dehydration stress affects Mayaro virus infection and vector competence in Aedes aegypti mosquitoes, finding that dehydration alters viral dissemination dynamics.

This study shows that repeated blood feeding during drought increases mosquito survival and viral transmission potential by enhancing activity and CO2 sensing.

This study identifies NS2B-55 and NS2B-65 variations as responsible for differences in NS2B-NS3 protease activity between JEV genotype I and III using fluorogenic peptide assays.

This study challenges the assumption that male mosquitoes exclusively feed on plant sugars by showing they can take blood under dehydration/starvation and transmit pathogens.

This paper demonstrates successful gene editing in Rhodnius prolixus using ReMOT Control and DIPA-CRISPR methods, achieving mutations in eye and cuticle color genes.

This study identifies putative nuclear localization and export signals in mosquito densovirus proteins using in silico analysis, enhancing understanding of viral protein trafficking and potential applications in mosquito control.

This study identifies and functionally characterizes IsAQP1, an aquaporin in Ixodes scapularis, showing it is a water channel with low glycerol permeability and salivary gland-specific expression.

The paper identifies and validates 106 miRNAs in Culex tarsalis mosquitoes using small RNA sequencing and experimental validation.

This study presents novel genome assemblies for four North American Anopheles mosquito species and identifies genes under positive selection that may contribute to their vectorial capacity.

The paper develops an integrated resource to analyze small RNA profiles in mosquitoes, revealing evolutionary dynamics and host responses to viruses and transposons.

The paper assembles and annotates a reference genome for Culex tarsalis, identifies genetic clusters, and estimates divergence time from Culex quinquefasciatus.

This study shows that actin bundles have different roles in shaping mosquito scales versus bristles, with actin bundles required for bristle elongation and scale width formation.

The paper develops a method to deliver CRISPR-Cas9 ribonucleoprotein into Nasonia vitripennis ovaries for germline gene editing using ReMOT or BAPC, achieving efficient somatic and germline editing.

This paper develops a CRISPR/Cas9 gene editing protocol for Bemisia tabaci by injecting adult females instead of embryos, enabling efficient heritable genome editing.

The paper adapts ReMOT Control to edit genes in Anopheles stephensi mosquitoes, achieving editing efficiency comparable to embryo injections.

This study investigates the role of Hsp70 in ZIKV infection, showing that Hsp70 facilitates viral entry and production.

This study shows dehydration increases blood feeding and activity in mosquitoes, likely to obtain water, and may enhance disease transmission.