BiochemistrybioRxivPreprint
CRISPR-Cas9 trans-cleavage is hindered by a closed R-loop, an elongated spacer, and an inactive HNH domain
Roser Montagud‐Martínez, Raúl Ruiz, Sara Baldanta…·Guillermo Rodrigo
The paper identifies structural factors affecting CRISPR-Cas9 trans-cleavage activity, including R-loop conformation, spacer length, and HNH domain activity.
DOI 10.1101/2025.04.17.649303