Synthetic BiologybioRxivPreprint
Optimized directed evolution of E. coli leucyl-tRNA synthetase adds many noncanonical amino acids into the eukaryotic genetic code including ornithine and Nepsilon-acetyl-methyllysine
Elise D. Ficaretta, Tarah J. Yared, Subrata Bhattacharjee…·Abhishek Chatterjee
This study optimizes a yeast-based directed evolution platform to expand the substrate scope of EcLeuRS, enabling incorporation of noncanonical amino acids like ornithine into mammalian cells.
DOI 10.1101/2024.11.27.625662