Paolo De Los Rios

The paper shows that alpha-synuclein phase separation can form Lewy body-like structures without pre-existing seeds, suggesting a new mechanism for neurodegeneration.

The paper introduces CLAPP, a method to determine paralog interactions by class, showing it can accurately assign interaction partners using weak or noisy scores when class labels are reliable.

The paper develops a self-consistent model to study phase separation and active processes in biomolecular condensates, showing how scaffold activity regulates phase behavior and enhances client transport near stability thresholds.

This paper introduces an Allosteric Logic Gate (ALG) that converts two independent deformations into a Boolean-like conformational output, demonstrating non-linear logic operations through optimized spring constants.

FeatureDCA improves protein sequence generation by conditioning DCA on principal components, achieving accurate structural and functional results across multiple families.

This paper describes a BSL-2 compatible purification protocol for full-length TDP-43 using refolding-assisted IMAC, enabling safe and reproducible production for in vitro studies.

This study investigates how Hsp110 enhances Hsp70's disaggregation activity through entropic pulling by increasing effective volume.

This study uses single-molecule nanopore experiments to demonstrate Hsp70 chaperones exert forces equivalent to 46 pN to extract trapped polypeptides, supporting the Entropic Pulling mechanism.

The paper investigates how J-domain proteins in yeast regulate Hsp70 chaperones through intramolecular interactions, showing auto-regulatory effects that reduce ATPase cycles.

The paper uses ANNs to classify J-domain proteins, revealing phylogeny, localization, and domain composition from sequence data, with insights into coevolution with Hsp70 partners.

This study uses a fluorescent reporter substrate to elucidate the stepwise mechanism of Hsp70 unfoldase action in converting aggregates into native proteins.

The paper applies direct coupling analysis to study proteins involved in iron-sulfur cluster biogenesis, revealing structural and interaction features of these proteins.

This study combines molecular simulations and co-evolutionary sequence analysis to model the Hsp70/Hsp40 interaction in bacteria, identifying a conserved interaction surface.