Na Ji

High Axial Resolution Is Necessary for Quantitative Two-Photon Calcium Imaging of Neuronal Populations

The paper shows that low axial resolution in two-photon calcium imaging corrupts neuronal activity measurements and that no analysis pipeline fully corrects these issues.

A red-emitting, genetically encoded indicator for two-photon voltage recording in vivo

This paper introduces VADER1, a red GEVI that enables reliable in vivo two-photon voltage imaging with millisecond resolution and supports dual-color imaging with calcium indicators.

Magnetogenetic control of endogenous calcium signaling via ROS-sensitive ion channels reveals astrocytic regulation of neural circuit activity

This paper demonstrates that magnetic control of astrocytic calcium signaling can modulate neural circuit activity, introducing a tool for wireless, cell-specific calcium regulation.

Designer indicators for two-photon recording of subthreshold voltage dynamics

The paper develops two new genetically encoded voltage indicators (JEDI3sub and JEDI3hyp) for improved two-photon recording of subthreshold voltage dynamics in vivo.

Adaptive optical correction for in vivo two-photon fluorescence microscopy with neural fields

NeAT improves in vivo two-photon imaging by correcting optical aberrations and motion without external training data.

A versatile, positive-going voltage indicator that enables accessible two-photon recordings in vivo

The paper introduces FORCE1s, a GEVI that enables robust two-photon voltage imaging in awake mice with high signal-to-noise and reliable spike detection.

In vivo aberration measurement and correction for ultrafast FACED two-photon fluorescence microscopy of the brain

This paper develops an adaptive optics method to correct optical aberrations in ultrafast two-photon microscopy, improving resolution and signal in live mouse brain imaging.

Convergence of Cortical and Thalamic Origins of Free Behavior Modulation of Mouse Primary Visual Cortex

The paper shows that mouse primary visual cortex integrates non-visual signals from top-down and stimulus-gated pathways during grating stimulation.

FACED 2.0 enables large-scale voltage and calcium imaging in vivo

The paper introduces FACED 2.0, a high-speed imaging technique that enables simultaneous voltage and calcium activity recording in vivo across large neuronal populations.

Retinal ganglion cell input to superior colliculus encodes salient information

This study shows that retinal ganglion cells encode salient visual features in the superior colliculus through their center-surround receptive fields.

Stimulation with ECoG electrodes modulates cortical activity and sensory processing in the awake mouse brain

This study shows that cortical surface electrical stimulation in awake mice initially activates L2/3 neurons and then causes prolonged inhibition, altering sensory processing and neuronal selectivity.

Parallel frequency-multiplexed aberration measurement for widefield fluorescence microscopy

This paper introduces PFAM and PFAM-SIFT methods for parallel aberration measurement in widefield fluorescence microscopy, enabling accurate correction of spatially varying aberrations in complex samples.

Structure and Function of Lens Suture Examined by 2-photon Fluorescence Microscopic Imaging

This study uses 2-photon fluorescence microscopy to examine lens suture structures in wildtype and KLPH-KO mice, revealing altered suture patterns and pathological features in the knockout model.

Suite3D: Volumetric cell detection for two-photon microscopy

Suite3D improves 3D cell detection in two-photon microscopy by correcting motion and segmenting cells across planes, enhancing detectability and signal quality.

Circuit-Based Understanding of Fine Spatial Scale Clustering of Orientation Tuning in Mouse Visual Cortex

The paper investigates spatial organization of orientation tuning in mouse visual cortex and identifies micro-clustered organization of orientation preference.

A dendritic mechanism for balancing synaptic flexibility and stability

This paper shows how cortical pyramidal neurons balance synaptic flexibility and stability by differentially regulating plasticity at oblique and basal dendrites.

Improving positively tuned voltage indicators for faster kinetics and higher contrast

The paper improves positively tuned voltage indicators by enhancing their kinetics and contrast for better spike detection.

maskNMF: A denoise-sparsen-detect approach for extracting neural signals from dense imaging data

maskNMF improves demixing of dense calcium imaging data by denoising, sparsening, and using constrained non-negative matrix factorization to accurately recover neural activity.

A positively tuned voltage indicator reveals electrical correlates of calcium activity in the brain

This paper introduces positively tuned GEVIs that enhance spike detection in vivo with standard imaging systems, offering higher temporal resolution than calcium indicators.

Retinal microvascular and neuronal pathologies probed in vivo by adaptive optical two-photon fluorescence microscopy

This study uses adaptive optical two-photon fluorescence microscopy to investigate retinal microvascular and neuronal pathologies in vivo, revealing microvascular lesions and pharmacological effects.

A distinct population of L6 neurons in mouse V1 mediate cross-callosal communication

The paper identifies a distinct population of L6 neurons in mouse V1 that mediate cross-callosal communication through anatomical and functional analysis.

Kilohertz two-photon fluorescence microscopy imaging of neural activity in vivo

This paper demonstrates ultrafast two-photon fluorescence microscopy for in vivo neural activity imaging at high spatiotemporal resolution in awake mice.

Penalized matrix decomposition for denoising, compression, and improved demixing of functional imaging data

~2-4x SNR gains and 20-300x compression on calcium and voltage imaging, from a patch-wise penalized matrix decomposition that splits signal from temporally uncorrelated noise without hyperparameter tuning.

Optical alignment device for two-photon microscopy

This paper introduces an optical alignment device to improve two-photon microscopy imaging by minimizing tilt angles between glass windows and the optical axis.

Three-photon fluorescence microscopy with an axially elongated Bessel focus

This paper demonstrates three-photon fluorescence imaging using an axially extended Bessel focus for imaging mouse brain slices and in vivo mouse brain imaging.

50 Hz volumetric functional imaging with continuously adjustable depth of focus

The paper introduces a CADoF Bessel beam module for volumetric functional imaging at 50 Hz volume rate in larval zebrafish.

Video-rate volumetric functional imaging of the brain at synaptic resolution

This paper introduces a method to achieve video-rate volumetric imaging of brain activity at synaptic resolution using Bessel foci in two-photon microscopy.