Molecular BiologybioRxivPreprint
Single- and duplex TaqMan-quantitative PCR for determining the copy numbers of integrated selection markers during site-specific mutagenesis in Toxoplasma gondii by CRISPR-Cas9
Kai Pascal Alexander Hänggeli, Andrew Frederick Hemphill, Norbert Müller…·Ghalia Boubaker
The paper develops single and duplex TaqMan qPCR methods to accurately quantify integrated selection markers in Toxoplasma gondii CRISPR-Cas9 knockouts.
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