Benjamin M. Woolston

This study uses 13C-MFA to analyze co-metabolism in Eubacterium limosum, finding that glucose cofeeding reduces flux through the Wood-Ljungdahl pathway and that cysteine provides gluconeogenic carbon.

This paper engineers Escherichia coli to produce or consume hydrogen sulfide in specific gut regions, demonstrating methods to increase and decrease H2S levels in mice.

This paper develops a transcriptional biosensor in E. coli to measure hydrogen sulfide (H2S) under both aerobic and anaerobic conditions, with a detection range of 50-750 μM.

This study uses AneVO to show that cofeeding C1 substrates with CO or glucose enhances growth and acetate productivity in E. limosum.

This study identifies Prevotella as a major source of sialidases in the vaginal microbiome, showing their prevalence and activity compared to Gardnerella.

This paper develops a recombineering system for Eubacterium limosum using RecT and Cas9 counterselection to enable markerless, scarless genomic modifications with high efficiency.