Benchmarking 16S rRNA gene amplicon analysis in high-diversity microbial communities reveals fundamental trade-offs in clustering and denoising
Cassandra Stamsaas, Torbjørn Rognes, Knut Rudi…·Hilde Vinje
This study benchmarks 16S rRNA gene analysis pipelines in high-diversity datasets, revealing trade-offs between clustering accuracy, species recovery, and abundance reconstruction.