Karen Verboom

This study benchmarks single cell RNA-seq technologies to assess their ability to detect transcriptional changes in NGP neuroblastoma cells after nutlin-3 treatment, finding heterogeneity in response not captured by bulk RNA-seq.

The paper presents a comprehensive dataset of TLX1 positive ALL-SIL lymphoblasts and primary T-ALL leukemias using polyA[+] and total RNA sequencing, ATAC sequencing, and ChIP sequencing, validated for quality and biological relevance.

The paper introduces a novel single cell strand-specific total RNA sequencing method that overcomes limitations of existing techniques and detects more genes, including non-polyadenylated ones.