Thorben Cordes

The paper quantifies mRNA and siRNA payload in lipid nanoparticles using fluorescence microscopy and FCS, showing size-dependent payload and coencapsulation dynamics.

This paper introduces a minimalistic 3D-printed setup for single-molecule FRET using blue-green dyes and photostabilizers, demonstrating its effectiveness for conformational studies.

The paper presents a systematic method for purifying fluorophore-labeled proteins using anion exchange chromatography, achieving high yields for FRET studies.

This paper develops a DNA-mediated photostabilization method for single-molecule and super-resolution imaging, showing improved performance at lower additive concentrations and enabling continuous replenishment of photostabilizers.

This study identifies a conserved lysine in TM5 of BasC as critical for cytoplasmic gate dynamics, showing its role in gating transitions and modulation by substrates and mutations.

This study introduces AXSI for measuring absolute intramolecular distances in proteins, validated against single-molecule FRET, showing its effectiveness under various solution conditions.

This study designs and synthesizes functional fluorophores using a scaffold-based Ugi reaction, enabling detection of reactive-oxygen species and controlled subcellular localization, with some compounds showing pyroptosis-inducing activity in cancer cells.

The paper introduces Brick-MIC, an affordable, open-source 3D-printed microscopy platform that enables single-molecule and super-resolution imaging techniques.

This paper develops a flow virometer using confocal fluorescence microscopy to rapidly and specifically detect single nanoparticles and viruses in microfluidic laminar flow.

The paper investigates the structural dynamics of SctV, a component of the Type III translocase export gate in E. coli, revealing its conformational changes and interactions with chaperones and ATPase.

ColiCoords is a Python package that analyzes bacterial fluorescence microscopy data by transforming Cartesian coordinates to cellular coordinates, enabling statistical analysis of single-cell data.