Author

Ayushman Dobhal

All India Institute of Medical SciencesORCID 0000-0002-8698-7197Unclaimed ORCID profile · follow to get notified of new preprints

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Synthetic Biology· 1

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Approach:experimental assay· 1 Organism:human· 1

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Kalpana LuthraiD· 1 shared Nitesh MishraiD· 1 shared Shaifali SharmaiD· 1 shared

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2020-2020 · peak 1/yr

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Kalpana LuthraORCID

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Synthetic BiologybioRxivPreprintApr 1, 2020

A versatile high throughput strategy for cloning the env gene of HIV-1

Nitesh Mishra, Ayushman Dobhal, Shaifali Sharma, Kalpana Luthra

This paper describes a high-throughput method for cloning HIV-1 env genes using Phusion polymerase and overlap extension PCR, enabling efficient analysis of diverse env genes from infected individuals.

DOI 10.1101/2020.04.01.020735

humanexperimental assay

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