Ana Paula Jacobus

EasyCAPS is a web tool that integrates dCAPS primer design with CRISPR-Cas9 strategies to improve genotyping and gene editing efficiency.

This paper introduces ReMaSSing, a method to identify and correct adaptive alleles in Saccharomyces cerevisiae for improved lignocellulosic hydrolysate tolerance through QTL mapping and breeding.

The paper introduces EasyGuide CRISPR, a CRISPR toolkit for engineering E. coli strains to grow on sucrose, and demonstrates its application in adaptive evolution experiments.

This study evolves yeast strains to tolerate ethanol shocks, finding that mutations improve stress survival but reduce growth, with some combinations offering better industrial performance.

The paper compares methods for simultaneously quantifying yeast and bacterial cells in mixed suspensions, finding that flow cytometry, Coulter counter, and Spread-plate methods are statistically similar for yeast, while microscopy and Drop-plate are similar for bacteria.

The paper introduces EasyGuide plasmids to simplify CRISPR/Cas9 gRNA assembly in yeast by enabling in vivo gRNA generation directly in yeast.

The paper compares genomes of Brazilian bioethanol Saccharomyces cerevisiae strains to show they form a unified group related to cachaca spirit yeasts.