Renata Sigrist

The paper introduces a type I-C CRISPR system, pCRISPR-Cas3, for efficient genome engineering in streptomycetes, outperforming Cas9-based systems in deletion and substitution capabilities.

The paper reports the discovery of maramycin, a novel isoquinolinequinone alkaloid produced through heterologous expression of a bifunctional enzyme in Streptomyces albidoflavus, showing moderate cytotoxicity against prostate cancer cells.

The paper uses independent component analysis to characterize the transcriptional regulatory network of Streptomyces albidoflavus and identifies iModulons that describe its activity across growth conditions.