João Carlos Lima

This paper reviews extremophilic polysaccharides' structure-function relationships in cryopreservation, identifying key traits for cold adaptation and their potential in designing non-toxic cryoprotectants.

This study shows that removing uronic acids from FucoPol reduces its cryoprotective activity, highlighting the importance of polyanionicity in ice growth disruption.

This paper explains how gel-forming polysaccharides protect cells from freezing damage through kinetic inhibition, Gibbs-Thomson modulation, and volumetric confinement mechanisms.

The paper evaluates 26 natural polysaccharides as cryoprotective agents, finding that fucose-rich polysaccharides significantly enhance post-thaw metabolic viability of Vero cells compared to other types.