Timothée Lionnet

The paper designs functional disordered regions in a transcription factor using biophysical principles to predict chromatin binding function.

The paper identifies a developmentally regulated long-range enhancer-promoter contact that is critical for SOX2 expression and neuroectodermal differentiation in human neural development.

ZATELLITE enables real-time visualization and manipulation of human centromeres in live cells using synthetic zinc fingers without causing DNA damage.

This study identifies cGAS-STING pathway activation as a key mechanism of inflammation in VEXAS, showing that UBA1 mutations disrupt ERAD and mitochondrial homeostasis, leading to STING-driven inflammation.

BeadBuddy corrects chromatic errors in single-molecule imaging data using fluorescent bead images, achieving sub-voxel resolution in DNA FISH and applicable across SMLM modalities.

This study uses a zebrafish model to identify genetic dependencies in melanocyte and melanoma development, revealing that gene loss can paradoxically promote aggressive phenotypes.

The paper uses polymer simulations to model chromatin dynamics and shows that productive interactions depend on contact frequency, capture radius, and duration, not just average contact matrices.

The paper shows that Integrator complex depletion disrupts histone gene expression timing by dispersing transcription machinery clusters.

RS-FISH detects FISH spots accurately and efficiently in 2D and 3D images, supporting applications like smFISH and spatial genomics.

This study uses live-cell imaging in yeast to show how PIC components explore the nucleus and assemble at promoters, revealing spatio-temporal coordination in transcription initiation.

The paper uses single-molecule imaging to study chromatin remodelers in budding yeast, revealing their fast kinetics and ATP-dependent dissociation from chromatin.

The paper uses live-cell single particle imaging to show that RNA polymerase II and Kin28/Cdk7 kinase are required for H2A.Z eviction in yeast, linking it to transcription processes.

The paper quantifies chromatin modification dynamics during zygotic genome activation in zebrafish embryos using fluorescent antibody imaging.

The paper introduces a smFISH method for single-mRNA detection in cleared Drosophila brains with subcellular resolution using a custom microscope.