Do Soon Kim

The paper designs and selects ribosomal active site mutants with high activity using a computational approach to overcome limitations in rRNA modification.

This study develops PERSIST-seq and In-line-seq to optimize mRNA stability and translation, finding that in-cell stability is more critical than ribosome load for protein output.

This study develops an in vitro method for ribosome synthesis and evolution called RISE, demonstrating its ability to select for ribosome variants resistant to clindamycin and revealing positive epistasis in successful genotypes.