Akiyoshi Ohashi

This paper introduces a new cultivation method that enhances microbial growth by maintaining pure cultures and enabling single-cell tracking, showing microbial interactions significantly improve cultivability.

The paper identifies a unique symbiotic relationship between Gracilibacteria and Zoogloea in activated sludge flocs using fluorescence in situ hybridization and metagenome-assembled genomes.

This study isolates previously uncultivated marine microbes using in situ cultivation and identifies growth initiation factors in sponge extracts that trigger resuscitation from dormancy.