Author

Nicholas J. Kapolka

University of North Carolina at Chapel HillORCID 0000-0003-2223-8207Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Pharmacology & Toxicology· 1 Synthetic Biology· 1

Signature Assays, Organisms & Methods

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Approach:benchmark study· 1 Assay:CRISPR screen· 1 Approach:deep learning· 1 Research Focus:drug response / drug discovery· 1 Approach:experimental assay· 1 Organism:human· 1 Research Focus:protein design· 1 Assay:protein structure· 1

Frequent Co-Authors

Andrew C. KruseiD· 1 shared Assaf AloniD· 1 shared Brian K. ShoichetiD· 1 shared Bruno ColoniD· 1 shared Bryan L. RothiD· 1 shared Daniel G. IsomiD· 1 shared Georgios SkiniotisiD· 1 shared Isabella GlenniD· 1 shared

Activity by Year

2024-2026 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 24 mutual co-author links

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1 shared paperPharmacology & Toxicology

Andrew C. KruseORCID

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2 papers

Recent·Most cited

Synthetic BiologyJournal of Biological ChemistryPeer-reviewedOct 1, 2026

Systematic mapping of chimera-permissive sites by CRISPR-guided PAM scanning

Jacinda Pujols, Kyutae David Lee, Nicholas J. Kapolka…·Daniel G. Isom

This paper uses CRISPR-guided PAM scanning to identify chimera-permissive sites in proteins, enabling the design of functional chimeras with preserved activity.

DOI 10.1016/j.jbc.2026.113627

yeasthuman

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Pharmacology & ToxicologySciencePeer-reviewedMay 16, 2024

AlphaFold2 structures template ligand discovery

Jiankun Lyu, Nicholas J. Kapolka, Ryan H. Gumpper…·Bryan L. Roth

This study evaluates AlphaFold2 models for ligand discovery, finding they perform similarly to experimental structures despite conformational differences, and identify potent agonists for the 5-HT2A receptor.

193 citationstop 1%

protein structuredeep learning

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