Shishir P. S. Chundawat

The paper uses N-GLYcanyzer to monitor Trastuzumab biosimilar N-glycoform changes in a microperfusion process, identifying key parameters affecting glycoform evolution.

This study investigates how mechanical pulling affects the unbinding of a cellulosomal carbohydrate-binding module from cellulose using simulations and experiments, showing that pulling orientation significantly impacts unfolding and unbinding dynamics.

The paper demonstrates that chemical rescue is a predictive proxy for glycosynthase activity, validated through correlation with enzymatic activity and transition state analysis.

This study shows that modifying the charge of PET-binding modules can enhance the thermostability and functional persistence of PET hydrolases.

This study improves cellulase activity through protein supercharging, identifying a variant with 2-3-fold higher activity on cellulosic substrates and demonstrating its synergistic potential with endocellulases.

This study determines the reaction kinetics of procainamide labeling of N-glycans to improve analytical workflows for glycoprotein manufacturing.

The paper identifies CSC and REM as mechanisms mediating plant cell resilience through cell wall-plasma membrane attachments under hyperosmotic stress.

This paper visualizes cellulose biosynthesis and microfibril network assembly in live Arabidopsis protoplasts using time-lapse imaging, revealing dynamics of fibril synthesis and network formation.

This paper expresses and characterizes family-2 glycosyltransferases using a cell-free system, showing their catalytic activity and kinetic properties.

This study uses acoustic force spectroscopy to measure rupture forces of CBM3a and CBM64 and their mutants, quantifying how mutations affect cellulose binding interactions.

This study shows that supercharging carbohydrate-binding modules enhances endocellulase activity, thermostability, and performance on cellulosic biomass.

The paper develops a pull-down binding assay to assess the directional binding preferences of cellulose-binding modules to cello-oligosaccharides, revealing similar affinity for cellohexaose but not cellopentaose.

The paper introduces a synthetic promoter-based azide biosensor toolkit for in vivo detection of azide anions in E. coli, offering tunable GFP expression and low-cost protein expression.