Chun‐Ting Liu

This paper identifies FoTO1 as a dual-function enzyme that enhances Taxol biosynthesis through catalytic conversion and non-catalytic organization of P450s in plants.

A multiplexed perturbation and single-nucleus RNA-seq screen of Taxus needles found eight new Taxol pathway genes, including FoTO1, enabling baccatin III and a late Taxol precursor in N. benthamiana.

This paper reconstitutes an early paclitaxel biosynthetic network by engineering Taxus genes to increase taxadien-5-ol accumulation and identify pathway byproducts.