Plant BiologyThe Plant JournalPeer-reviewed
A normalization method that controls for total RNA abundance affects the identification of differentially expressed genes, revealing bias toward morning-expressed responses
Kanjana Laosuntisuk, Amaranatha R. Vennapusa, Impa M. Somayanda…·Colleen J. Doherty
The paper evaluates how normalization methods affect differential gene expression analysis in sorghum, showing that spike-in controls reveal biases in gene expression related to time of day and chilling stress.
17 citations