A synthetic cell phage cycle
Antoine Lévrier, Paul Soudier, David Garenne…·Vincent Noireaux
This study creates a synthetic cell-free system where T7 phages infect synthetic cells, demonstrating a complete viral life cycle in vitro.
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Centre National de la Recherche ScientifiqueORCID 0009-0002-1649-8328Unclaimed ORCID profile · follow to get notified of new preprints
4 papers
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Antoine Lévrier, Paul Soudier, David Garenne…·Vincent Noireaux
This study creates a synthetic cell-free system where T7 phages infect synthetic cells, demonstrating a complete viral life cycle in vitro.
Antoine Lévrier, Julien Capin, Pauline Mayonove…·Jerome Bonnet
This paper introduces minireporters for monitoring gene expression and peptide production in cell-free systems, reducing DNA length and synthesis costs while maintaining reliable output.
Antoine Lévrier, Ioannis Karpathakis, Bruce Nash…·Vincent Noireaux
The paper introduces PHEIGES, a method to rapidly engineer and select phages using cell-free transcription-translation, producing high titers of engineered phages within one day.
Angelo Cardoso Batista, Antoine Lévrier, Paul Soudier…·Manish Kushwaha
This paper develops exonuclease-deficient cell-free extracts and optimized buffers to enable robust expression from unprotected linear DNA without additional protection strategies.