David Fange

The paper develops a method for in situ genotyping of bacterial libraries using chromosomally expressed barcodes to measure intracellular maturation times of red fluorescent proteins.

This study investigates how mutations in the hinge region of LacI affect binding stability and inducibility, showing a trade-off between binding strength and inducibility rather than speed and stability.

This study evaluates if phenotypic antibiotic susceptibility testing can be performed on single bacterial cells by monitoring phenotypic changes over time, finding that single-cell responses are heterogeneous and require sequential antibiotic exposure for accurate resistance classification.

This study uses live-cell imaging of fluorescently labeled loci in E. coli to inform a dynamic polymer model of chromosome organization across the cell cycle, revealing global reorganization of the chromosome structure.

This study uses fluorescence microscopy to show that Escherichia coli replisomes are stationary and chromosomal loci move toward them during replication.

The paper demonstrates in situ genotyping of pooled strain libraries by combining single molecule fluorescence imaging with barcoded plasmids to identify individual cell genotypes after phenotype characterization.