Willemijn S. de Voogt

This paper develops a modular strategy for delivering CRISPR-Cas9 via extracellular vesicles using aptamer-based loading and UV-activated cargo release, demonstrating efficient delivery and versatility in Cas9 variants.

This study introduces a metabolic labeling method using 5-EU and UCK2 overexpression to track RNA in donor cells, EVs, and recipient cells, enabling visualization and quantification of EV-RNA transfer.

EV-Elute enables the isolation and functional comparison of surface marker-defined extracellular vesicle subpopulations using a universal, non-destructive method.

This study investigates how hypoxia and TNF-alpha affect extracellular vesicle release from hiPSC-CMs under stress conditions, identifying nSmase2 and mTOR signaling pathways involved.