Acoustic wave-induced stroboscopic optical mechanotyping of adherent cells

In this study, we present a novel, high content technique using an innovative cylindrical acoustic transducer, stroboscopic fast imaging and homodyne detection to recover the mechanical properties (dynamic shear modulus) of living adherent cells at low ultrasonic frequencies. By analyzing the micro-oscillations of cells we were able to simultaneously mechanotype whole populations of cells with sub-cellular resolution. The technique can be combined with standard fluorescence imaging allowing to further cross-correlate biological and mechanical information. We demonstrate the potential of the technique by mechanotyping co-cultures of different cell types with significantly different mechanical properties.