Tissue embedding in a silica hydrogel for functional investigations
Fabio MiazziiD, Sabine Kaltofen, Jan E. BelloiD, Bill S. HanssoniD, Dieter WicheriD
Abstract
Embedding procedures can be challenging for delicate specimens. We present a method based on a sodium metasilicate (waterglass) gel to embed tissue samples for acute physiological studies. We show that the application of such a colloidal gel has minimal effects on the properties of buffered solutions and cell activity, allowing functional investigations on sensitive cells such as ciliated insect olfactory neurons.
02 /Specification & Classification
Click any tag to browse matching papers- Citations
- 0
- Approach
Reproducibility ledger
Nobody has reported trying to reproduce this paper yet. Did you replicate an experiment or run the code? Your report saves the next lab a week.
Sign in to file a reproducibility report.
Related papers
- A simple device to immobilize protists for electrophysiology and microinjection
A. Kulkarni, N. Escoubet, … R. Brette·Journal of Experimental BiologyPeer-reviewed·Jun 17, 2020
- An actuated neural probe architecture for reducing gliosis-induced recording degradation
T. L. Massey, L. S. Kuo, … M. M. Maharbiz·bioRxiv·Jul 30, 2018
- Evaluation of Silica and Bioglass Nanomaterials in Pulp-Like Living Materials
Daline Mbitta Akoa, Anthony Avril, … Thibaud Coradin·ACS Biomaterials Science & EngineeringPeer-reviewed·Jan 13, 2025
- Hydrogel encapsulation of living organisms for long-term microscopy
K. Burnett, E. Edsinger, D. R. Albrecht·Communications BiologyPeer-reviewed·Jun 21, 2018
- Intracortical probe arrays with silicon backbone and microelectrodes on thin polyimide wings enable long-term stable recordings in vivo
Antje Kilias, Yu‐Tao Lee, … Patrick Ruther·Journal of Neural EngineeringPeer-reviewed·Nov 15, 2021
- First Biodegradable Bioactive Glass-based Humidity Sensor
A. Gharbi, A. Y. Kallel, … N. Ashammakhi·bioRxiv·Dec 6, 2022