bioRxivDevelopmental BiologyPreprint
Verified the effectiveness of AsCpf1 system in a variety of vertebrate species
Zhuo LiiD, Zhaoying ShiiD, Nana FaniD, Yongqiang CheniD, Jing GuoiD, Jingchun WuiD, Hong SongiD, Shilong Chu, Kunlun Mo, Bentian Zhao, Zhen Ouyang, Dandan Tian, Shaoyang Zhao, Jieying ZhuiD, Jiekai CheniD, Yonglong CheniD, Liangxue LaiiD, Duanqing PeiiD
Abstract
CRISPR/Cpf1 system is a novel genomic editing tool. Because of its more sophisticated components, and lower off-target rate, it has the potential to become a better gene-editing tool. Previous reports showed that CRISPR/Cpf1 could work effectively in multiple species. But our data show that AsCpf1 activity has a big difference in different vertebrates. Using in vitro experiments, we finally learned that the difference between species is due to temperature.
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