A chemical strategy to control protein networks in vivo

Michael J. Ziegler; Klaus Yserentant; Valentin Dunsing; Volker Middel; Antoni J. Gralak; Kaisa Pakari; Jörn Bargstedt; Christoph Kern; Annett Petrich; Salvatore Chiantia; Uwe Strähle; Dirk‐Peter Herten; Richard Wombacher

doi:10.1038/s41589-021-00922-3

Synthetic BiologyNature Chemical BiologyDec 21, 2021

Peer-reviewed journal article

A chemical strategy to control protein networks in vivo

Michael J. Ziegler iD, Klaus Yserentant iD, Valentin Dunsing iD, Volker Middel, Antoni J. Gralak iD, Kaisa Pakari iD, Jörn Bargstedt iD, Christoph Kern, Annett Petrich iD, Salvatore Chiantia iD, Uwe Strähle iD, Dirk‐Peter Herten iD, Richard Wombacher iD

Abstract

Direct control of protein interaction by chemically induced protein proximity (CIPP) holds great potential for cell- and synthetic biology as well as therapeutic applications. However, toxicity, low cell-permeability and lack of orthogonality currently limit the use of available chemical inducers of proximity (CIP). We present Mandi, a novel CIP and demonstrate its applicability in cell culture systems as well as living organisms for protein translocation, protein network shuttling and manipulation of endogenous proteins.

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