A new group of LysM-RLKs involved in symbiotic signal perception and arbuscular mycorrhiza establishment

Yi Ding; Zélie Lesterps; Virginie Gasciolli; Anne-Laure Fuchs; Mégane Gaston; Lauréna Medioni; Annélie de-Regibus; Céline Remblière; Céline Vicédo; Sandra Bensmihen; Jean‐Jacques Bono; Julie V. Cullimore; Guilhem Reyt; Marion Dalmais; Christine Saffray; Solène Mazeau; Abdelhafid Bendahmane; Richard Sibout; Michiel Vandenbussche; Jacques Rouster; Tongming Wang; Guanghua He; Arnaud Masselin; Sylvain Cottaz; Sébastien Fort; Benoît Lefebvre

doi:10.1038/s41467-025-60717-1

Plant BiologyNature CommunicationsJul 1, 2025

Peer-reviewed journal article

A new group of LysM-RLKs involved in symbiotic signal perception and arbuscular mycorrhiza establishment

Yi Ding iD, Zélie Lesterps iD, Virginie Gasciolli iD, Anne-Laure Fuchs, Mégane Gaston, Lauréna Medioni iD, Annélie de-Regibus, Céline Remblière, Céline Vicédo, Sandra Bensmihen iD, Jean‐Jacques Bono iD, Julie V. Cullimore iD, Guilhem Reyt iD, Marion Dalmais iD, Christine Saffray, Solène Mazeau iD, Abdelhafid Bendahmane iD, Richard Sibout iD, Michiel Vandenbussche iD, Jacques Rouster iD, Tongming Wang iD, Guanghua He iD, Arnaud Masselin iD, Sylvain Cottaz iD, Sébastien Fort iD, Benoît Lefebvre iD

Abstract

Lipo-chitooligosaccharides (LCO) and short-chain chitooligosaccharides (CO) are produced by arbuscular mycorrhizal fungi (AMF) and activate the plant symbiosis signalling pathway, which is essential for mycorrhiza formation. High affinity LCO receptors belonging to the LysM receptor-like kinase (LysM-RLK) phylogenetic group LYR-IA play a role in AM establishment, but no plant high affinity short-chain CO receptors have yet been identified. Here we studied members of the uncharacterized LYR-IB group, and found that they show high affinity for LCO, short- and long-chain CO, and play a complementary role with the LYR-IA LCO receptors for AM establishment. While LYR-IB knock out mutants had a reduced AMF colonization in several species, constitutive/ectopic expression in wheat increased AMF colonization. LYR-IB function is conserved in all tested angiosperms, but in most japonica rice a deletion creates a frameshift in the gene, explaining differences in AM phenotypes between rice and other monocot single LYR-IA mutants. In conclusion, we identified a class of LysM-RLK receptors in angiosperms with new biochemical properties and a role in both LCO and CO perception for AM establishment.

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