CUT&ID for simultaneous profiling of protein-DNA and protein-protein interactions

We developed CUT&ID to simultaneously profile the native genomics and proteomics associations of any endogenous target, in a single streamlined workflow with no need of transgenesis. CUT&ID is enabled by a fusion protein that drives sequential target recognition, proximal protein biotinylation and DNA cleavage in living cells. CUT&ID is broadly applicable and yields high signal-to-noise genomics and proteomics identification across all types of gene regulators tested.