Pre-existent adult sox10+ cardiomyocytes contribute to myocardial regeneration in the zebrafish

Marcos Sande-Melón; Ines Joao Marques; María Galardi‐Castilla; Xavier Langa; María Pérez-López; Marius Alexandru Botos; Gabriela Guzmán; David Miguel Ferreira Francisco; Dinko Pavlinić; Vladimı́r Beneš; Rémy Bruggmann; Nadia Mercader

doi:10.1101/662536

Developmental BiologybioRxivJun 7, 2019

Preprint

Pre-existent adult sox10+ cardiomyocytes contribute to myocardial regeneration in the zebrafish

Marcos Sande-Melón iD, Ines Joao Marques iD, María Galardi‐Castilla iD, Xavier Langa iD, María Pérez-López, Marius Alexandru Botos iD, Gabriela Guzmán iD, David Miguel Ferreira Francisco iD, Dinko Pavlinić iD, Vladimı́r Beneš iD, Rémy Bruggmann iD, Nadia Mercader iD

Abstract

During heart regeneration in the zebrafish, fibrotic tissue is replaced by newly formed cardiomyocytes derived from pre-existing ones. It is unclear whether the heart is comprised of several cardiomyocyte populations bearing different capacity to replace lost myocardium. Here, using sox10 genetic fate mapping, we identified a subset of pre-existent cardiomyocytes in the adult zebrafish heart with a distinct gene expression profile that expanded massively after cryoinjury. Genetic ablation of sox10+ cardiomyocytes severely impaired cardiac regeneration revealing that they play a crucial role for heart regeneration.

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