Versatile polyketide biosynthesis platform for production of aromatic compounds in yeast

Tadas Jakočiūnas; Andreas Klitgaard; David Romero-Suárez; Christopher J. Petzold; Jennifer  W. Gin; Yaojun Tong; Rasmus John Normand Frandsen; Tilmann Weber; Sang Yup Lee; Michael Krogh Jensen; Jay D. Keasling

doi:10.1101/618751

Synthetic BiologybioRxivApr 25, 2019

Preprint

Versatile polyketide biosynthesis platform for production of aromatic compounds in yeast

Tadas Jakočiūnas iD, Andreas Klitgaard iD, David Romero-Suárez iD, Christopher J. Petzold iD, Jennifer W. Gin iD, Yaojun Tong iD, Rasmus John Normand Frandsen iD, Tilmann Weber iD, Sang Yup Lee iD, Michael Krogh Jensen iD, Jay D. Keasling iD

Abstract

To accelerate the shift to bio-based production and overcome complicated functional implementation of natural and artificial biosynthetic pathways to industry relevant organisms, development of new, versatile, bio-based production platforms is required. Here we present a novel yeast platform for biosynthesis of bacterial aromatic polyketides. The platform is based on a synthetic polyketide synthase system enabling a first demonstration of bacterial aromatic polyketide biosynthesis in a eukaryotic host.

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