Genome sequencing of the Monkeypox virus 2022 outbreak with Amplicon-based Oxford Nanopore MinION sequencing
Annika BrinkmanniD, Katharina Pape, Steven Uddin, Niklas Woelk, Sophie Förster, Heiko K. Jessen, Janine MicheliD, Claudia KohliD, Lars SchaadeiD, Andreas NitscheiD
Abstract
We present an amplicon-based assay for MinION Nanopore sequencing of monkeypox virus genomes from clinical specimens, obtaining high-quality results for up to 99 % of the genomes, even for samples with high Ct values of up to 30 which are not suitable for shotgun sequencing.
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