Gene editing of the multi-copy H2A.B gene family by a single pair of TALENS

Nur Diana Anuar; Matthew A. Field; Sebastian Kurscheid; Lei Zhang; Edward J. Rebar; Philip D. Gregory; Josephine Bowles; Peter A. Koopman; David John Tremethick; Tatiana Soboleva

doi:10.1101/233379

GenomicsbioRxivDec 13, 2017

Preprint

Gene editing of the multi-copy H2A.B gene family by a single pair of TALENS

Nur Diana Anuar iD, Matthew A. Field iD, Sebastian Kurscheid iD, Lei Zhang iD, Edward J. Rebar iD, Philip D. Gregory iD, Josephine Bowles iD, Peter A. Koopman iD, David John Tremethick iD, Tatiana Soboleva iD

Abstract

In view of the controversy related to the generation of off-target mutations by gene editing approaches, we tested the specificity of TALENs by disrupting a multi-copy gene family using only one pair of TALENS. We show here that TALENS do display a high level of specificity by simultaneously knocking out the function of the three genes that encode for H2A.B.3. This represents the first described knockout of this histone variant.

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