microscopy & imaging analysis

The paper identifies ER-rich conduits formed by peripheral axons and breast cancer cells, revealing a structural interface for potential intercellular communication.

This study uses optical clearing and light-sheet fluorescence microscopy to visualize 3D microanatomy of human head-and-neck tissues and correlate it with conventional histology.

The paper shows that dermal-epidermal junction geometry influences stem cell fate in mouse skin, with concave areas promoting proliferation and convex areas promoting differentiation.

The paper analyzes brain-wide neural activity in C. elegans during olfactory navigation and identifies functional circuits linking sensory input to motor behavior across different environments and behavioral states.

The paper uses 3D super-resolution tracking to show that Bacteroidota gliding involves multiple open filament tracks, challenging the single closed loop model.

The paper identifies a specialized cell layer in rice stems that functions as a gravity sensor, distinct from the lignified endodermis, and shows its role in gravitropic responses through starch sedimentation and auxin signaling.

This study combines live-cell imaging and mathematical modeling to show how cancer cells adapt to stress in spatially confined environments, revealing cooperative and competitive strategies influenced by spatial organization and microenvironment dynamics.

The paper uses advanced imaging techniques to study how multiple organelles coordinate during T cell killing, revealing a calcium-driven process of organelle assembly and movement.

The paper shows that single-molecule bouncing at a presynaptic interface enables protein enrichment without slowing diffusion.

GraphExperiment provides an R/Bioconductor package to store and manipulate high-dimensional quantitative data alongside associated networks in a unified framework.

This study uses MINFLUX nanoscopy to show nuclear speckles are hierarchically organized into dynamic nanoscale interaction networks that regulate molecular dynamics.

The paper investigates how biomechanical stress in the retinal pigment epithelium (RPE) contributes to the enlargement of laser scars over time using imaging and histological analysis.

This study shows that sharks and rays have cartilage canals in their adult skeletons, which are vascular but do not trigger endochondral bone formation, suggesting an ancient role in nutrient transport.

This study uses advanced imaging techniques to clarify SUN5's localization and dynamics during spermiogenesis, resolving previous controversies about its structure and function.

The paper identifies CYLD as a host deubiquitinase recruited to Listeria monocytogenes surface via InlC and RNF213, which facilitates cell-to-cell spread in epithelial cells.

The paper describes protocols for calcium imaging using a genetically encoded biosensor and fluorescence lifetime imaging to improve measurement robustness.

This study determines the structure of human GluD1 and elucidates its ligand-gating mechanism using cryoEM and electrophysiology.

This study identifies kinetic differences in RAS-BRAF engagement between KRAS and NRAS, showing how oncogenic mutations and inhibitor sensitivity are influenced by these dynamics.

This study characterizes the anatomy of Arabidopsis hydathodes using imaging techniques, revealing a dense and heterogeneous epithem involved in apoplastic fluid release.

This study uses HS-AFM to show yeast TOM complexes predominantly form trimers that dynamically dissociate into dimers and monomers, revealing structural and dynamic features of the TOM complex.

The paper develops a time-space resolved fluorescence spectroscopy technique to study light-harvesting dynamics in Chlamydomonas cells under state transitions.

The paper investigates nuclear wrinkles in epithelial cells, finding they form due to geometric adaptations to mechanical stress and cell shape changes, without causing damage.

This paper introduces a label-free method using amide I kurtosis from CARS microscopy to detect cardiac amyloid in human tissue, showing high spatial correlation with Congo red staining.

The paper identifies extensive membrane contact sites between the mealybug endosymbiont Tremblaya and host mitochondria, suggesting these interactions provide essential membrane and metabolic components.

The paper compares 3D vascular imaging modalities to assess their accuracy in quantitative measurements and computational modeling of vascular morphology.

This study uses advanced microscopy to characterize coccolith formation in Gephyrocapsa huxleyi, revealing how crystal growth is influenced by spatial constraints.

This study shows macrophages can distinguish and sort pathogenic bacteria and apoptotic material during phagocytosis, using distinct engulfment strategies.

The paper shows that OspA antibodies rapidly stop B. burgdorferi motility, using single-cell fluorescence microscopy.