Bacterial Genetics and Biotechnology

This study shows that diverse stresses cause periplasmic expansion in E. coli through distinct mechanisms, but UDP-glucose synthesis is required for expansion under some conditions.

The paper describes a tetracycline-inducible promoter, PtetB, from Acinetobacter baumannii, showing high induction and low background expression in multiple Gram-negative bacteria.

This study identifies GndA, a microprotein in Escherichia coli, which promotes cell growth during heat shock independently of its overlapping 6PGD protein.

The paper identifies a molecular clutch mechanism in Borrelia burgdorferi flagellar motors that controls stator engagement and torque generation through distinct conformational states.

This study uses cryo-EM to determine the structure of a light-regulated bacterial two-component system, revealing how light affects signaling through changes in histidine kinase and response regulator interactions.

The paper identifies three periplasmic enzymes in E. coli that release polysaccharide cargo from the lipid carrier undecaprenyl, revealing a mechanism for bacterial envelope biogenesis and potential antibiotic targets.

This study shows KhpA and KhpB in Campylobacter jejuni depend on each other for RNA recognition, with KhpA using a KH domain and KhpB an atypical R3H domain.

This study identifies a novel regulatory mechanism for the LytM/M23 family endopeptidase LmdC in P. aeruginosa, showing it is auto-cleaved and degraded by proteases Prc and CtpA, with inhibition by Baf and Api.

This paper uses ICA to analyze TraDIS-Xpress data from E. coli, identifying functional modules of antimicrobial resistance genes across multiple stress conditions.

The paper identifies gain-of-function mutations in RodZ that enhance Rod system activity in E. coli by mimicking FtsN's role in divisome activation.

This study investigates how Crl regulates gene transcription in E. coli during exponential growth, both independently and in conjunction with RpoS, under varying environmental conditions.

This study shows that the bacterial ompW gene family expands through duplication and horizontal transfer, enabling functional diversification during host adaptation.

The paper shows that Fusobacteriaceae bacteria use a plasmid-derived replication initiator protein, RPA, instead of the chromosomal dnaA gene, displacing the ancestral system.

This study establishes genetic tractability in Jeongeupia sacculi HS-3 by disrupting rpoS, showing it regulates biofilm formation under low-nutrient conditions but not general stress survival.

The paper uses phylogenetic analysis to show that bacterial σ-factors evolved from single-domain DNA-binding transcription factors through domain fusion.

The paper identifies two TBDTs in Marinobacter nauticus that mediate Fe-petrobactin uptake, showing distinct regulatory mechanisms and evolutionary trajectories, and demonstrates Fe-petrobactin's role in stimulating prokaryotic growth in Southern Ocean communities.

The paper identifies a branched RNA stem-loop (hp3) as a major regulator of RNase E synthesis in Escherichia coli, showing it binds RNase E with high affinity and functions additively with hp2.

The paper shows that routine strain handling of Komagataeibacter rhaeticus exposes recurrent genome reduction at a dynamic accessory locus, leading to phenotypic variation.

This paper shows BR-bodies in Brucella regulate RNA stability and virulence by linking phase separation to stress resistance and intracellular fitness.

This study shows bacteria hyperpolarize when oxygen levels drop, linking membrane potential to oxygen stress and antibiotic susceptibility.

This study investigates how CopY, a bacterial copper regulator, uses two non-equivalent operator sites to repress gene expression through distinct molecular mechanisms.

This study reveals how IdeR distinguishes DNA sequences by applying strain to non-cognate DNA through differential dielectric clefts.

This study identifies SroA as a regulator linking SigS-dependent stress signaling to metabolic remodeling in S. aureus by altering gene expression related to nitrate respiration and nucleotide biosynthesis.

This study identifies minJ and divIVA as essential for chromosome segregation in Bacillus subtilis when SMC function is impaired, highlighting the interdependence between chromosome segregation and cell division.

This study identifies MgpB and CorB as new mediators of magnesium homeostasis in Salmonella, showing they reduce intracellular Mg2+ and protect against excess-cation stress.

This study identifies IscR-mediated morphological changes in A. baumannii that reduce stress molecule uptake, enhancing virulence and survival under oxidative stress and antibiotic treatment.

This study shows MinC modulates MinD membrane release and oscillation period in E. coli by priming MinD for MinE-dependent release, affecting division machinery placement.

This study identifies two D-loop resolution systems in bacteria, YraN/ComM and CoiA/RadA, that work together to process the transformation D-loop during natural genetic transformation.

The paper shows that Achromatium bacteria have genetically diverse chromosomes within cells, inherited by daughter cells, and that spatial segregation of chromosomes drives genomic variability.