Bacterial Genetics and Biotechnology

This paper develops a standardized toolkit for predictable and tunable protein secretion and surface display in Bacteroides thetaiotaomicron by optimizing lipoprotein signal peptides.

The paper identifies a mechanism where a small RNA guides a protein regulator to target mRNAs, enabling programmable post-transcriptional control in bacteria.

This study identifies a PP2A regulatory network in Sulfolobus acidocaldarius that controls surface structures and motility through phosphorylation-dependent signaling.

This study shows that the RND efflux pump FemT in Staphylococcus aureus helps maintain lipid homeostasis by exporting isoprenoid and carotenoid lipids, preventing oxidative damage.

The paper develops a pipeline to identify environment-responsive promoters in gut-resident Bacteroidaceae by mining transcriptomes and screening biosensors in vivo.

This paper shows TrbA interacts with KorB to lock it as a transcriptional repressor on multi-drug resistance plasmids, using a conserved aromatic interface.

This study identifies RodZ's role in activating the elongasome in E. coli by interacting with MreB and MreCD, switching MreBCD to an active state to stimulate PG synthesis.

This study investigates the role of H-NS repression in virulence gene expression and pathogen adaptation by using a Salmonella strain with reduced H-NS DNA-binding affinity.

This study investigates how glutathione import affects zinc tolerance in Streptococcus mutans, revealing a balance between protection and toxicity through a Trojan horse mechanism.

This paper shows YpeB inhibits SleB in dormant Bacillus subtilis spores through direct interaction, preventing premature cortex degradation.

This study identifies distinct roles for CetZ1 and CetZ2 in shaping Haloferax volcanii cell morphology during different growth phases, showing CetZ2 maintains plate shape in stationary phase by counteracting CetZ1's rod-forming activity.

This study shows that monounsaturated fatty acid biosynthesis is essential for streptococcal growth, stress tolerance, and virulence, with species-specific effects on pathogenesis.

The paper examines the leading region of conjugative plasmids and finds evidence of sequence adaptation for translational efficiency in anti-defense genes.

This paper analyzes the evolution and functional diversification of the WhiG sigma factor in Actinomycetota, revealing two distinct clades with different regulatory mechanisms and expanded biological roles.

The paper shows that integrative and conjugative elements in Novosphingobium aromaticivorans facilitate frequent chromosomal recombination between strains but not between species.

This paper investigates polyP metabolism in Bacillus cereus, identifying two distinct polyP pools: an intracellular PPK1-dependent pool and an extracellular pool synthesized via an unknown pathway.

This study shows that MazF toxin in Gram-positive bacteria specifically cleaves mRNAs to regulate stress response and enable rapid regrowth.

The paper shows that deleting rpmJ in E. coli increases zntA transcription via a translation-dependent mechanism involving early zntA mRNA translation.

This study reports cryo-EM structures of BAM-BepA complexes and identifies loop conformations that regulate BepA's dual function in substrate triage.

This study uses genome shuffling in Bacillus subtilis to map quantitative trait loci for complex phenotypes, identifying high-confidence QTLs of moderate size.

This study identifies genes critical for acid and oxidative stress tolerance in Streptococcus mutans using CRISPRi screens and reveals core physiological processes involved in stress adaptation.

This paper defines the genome-wide CosR regulon in Campylobacter jejuni using ChIP-seq and DNase I footprinting, revealing its role in transcriptional control and redox-dependent regulation.

This paper identifies a hierarchical regulatory cascade in Staphylococcus aureus that defines the temporal window for natural transformation by linking environmental and metabolic cues to gene expression.

This study quantifies surplus of core biosynthesis components in E. coli and shows it has no cost to steady-state growth, instead aiding adaptation and reducing cell death.

The paper identifies candidate adaptive modules in Leclercia barmai associated with 3-NPA utilization through genomic and transcriptomic analysis.

This study shows OmrA sRNA inhibits Ppc translation, reducing TCA cycle flux and impairing bacterial growth, with implications for RNA-mediated metabolic regulation.

This paper identifies the TmaT-AftD interaction as essential for mycolate transport via the CmpL4 pathway and AG synthesis in Corynebacterium glutamicum.

This study identifies a c-di-AMP-controlled glutamine synthesis pathway critical for survival of Staphylococcus aureus TD-SCVs in the thymidine-limited CF lung.

This study investigates ParBF biocondensate dynamics in bacteria, showing they maintain stability through regulated phase separation and ParAF's dual role in assembly and coalescence.