Biofuel production and bioconversion

Understanding Substrate Transport and Metabolism in Clostridium thermocellum to Improve Biofuel Production

This study identifies substrates that C. thermocellum can transport and metabolize, revealing insights into its redox balance and fermentation product formation for biofuel production.

Engineering anaerobic fungal-bacterial consortia for direct conversion of lignocellulosic biomass into medium-chain fatty acids

This study engineers an anaerobic fungal-bacterial consortium to convert lignocellulosic biomass into medium-chain fatty acids without pretreatment, achieving a 21.0% conversion yield.

Taming complexity in enzymatic saccharification: A predictive hierarchical modeling framework

The paper develops a hierarchical modeling framework to predict enzymatic saccharification dynamics by simplifying complex interactions through successive model reductions.

Comparative sugar utilisation and metabolism of mannose as co-substrate indicate flexibility in carbon metabolism in anaerobic gut fungi

This study compares sugar utilization and metabolism in anaerobic gut fungi, revealing species-specific responses to co-substrates and highlighting functional diversity in carbon metabolism.

Sugar-mediated inhibition of growth and lignocellulose degradation in anaerobic gut fungi revealed using cellulose filter paper

This study investigates how free sugars and their polymeric forms affect growth and lignocellulose degradation in anaerobic gut fungi using cellulose filter paper as a model.

OxyR regulates the oxidative stress response in Zymomonas mobilis during oxic growth and anoxic biofuel fermentation

This study shows that OxyR regulates oxidative stress in Zymomonas mobilis during oxic growth and anoxic fermentation, with its absence impairing growth and ethanol production in hydrolysate.

Surface Binding Sites Orchestrate Long-Range Control of Active-Site Dynamics and Glucose Tolerance in β-glucosidase, BglB

This study identifies surface secondary binding sites in β-glucosidase BglB that regulate glucose inhibition and catalytic activity through long-range structural and energetic changes.

One-pot lactic acid production from rice straw: A consolidated bioprocess with enzymatic pretreatment-saccharification and Microbial co-fermentation

This paper develops a one-pot lactic acid production process from rice straw using enzymatic pretreatment and microbial co-fermentation, achieving high yield and purity.

Production of cellobiose from ionic liquid-treated cellulose using the highly thermostable cellobiohydrolase HmCel6A-3SNP at 80°C and analysis of enzymatic accessibility to the substrate

This study produces cellobiose from ionic liquid-treated cellulose using a thermostable enzyme at 80°C, achieving higher yields and purity through optimized pretreatment and enzyme conditions.

Comparative Secretome Analysis and Enzyme Cocktail Optimization of Six Fungal Species Under Solid-State and Submerged Fermentation for Lignocellulosic Saccharification of Flax Shives

This paper compares fungal secretomes and enzyme cocktails under different fermentation modes to optimize lignocellulosic saccharification of flax shives.

Interrelationship of substrate crystallinity, enzyme binding strength, and cellulase activity

This study examines the relationship between substrate crystallinity, enzyme binding strength, and cellulase activity, revealing a linear free energy relationship with varying slopes linked to substrate crystallinity.

Probing the role of residues lining the active site in the generation of glucose-tolerant variants of a fungal GH1 enzyme

This study identifies glucose-tolerant variants of a fungal β-glucosidase through site-specific mutations, improving its industrial applicability by enhancing glucose tolerance and kinetic properties.

There is an I in team: individual improvements in supercharged cellulase cocktail facilitates cooperative cellulose degradation

This study improves cellulase activity through protein supercharging, identifying a variant with 2-3-fold higher activity on cellulosic substrates and demonstrating its synergistic potential with endocellulases.

Exploring differential interactional preferences of enzyme-bearing dockerins for cohesin domains in the Clostridium thermocellum cellulosome

The paper examines differential interactional preferences between cohesin and dockerin domains in the cellulosome of Clostridium thermocellum, identifying specific binding affinities and modes.

DYNAMICS OF CONTAMINANT MICROBES IN BIOETHANOL PRODUCTION FROM SUGARCANE

The paper investigates microbial contaminants in sugarcane bioethanol production, finding dynamic bacterial communities affect ethanol yield and yeast viability.

A funnel-type pipeline for accelerating discovery of tailored designer xylanosomes for efficient arabinoxylan valorization

The paper develops a funnel-type pipeline for designing and testing designer xylanosomes to efficiently break down arabinoxylan, identifying optimal variants for biomass valorization.

A Highly Thermostable and Novel GH5 Endoglucanase from Bacillus sp. Strain BS with Enhanced Biomass Saccharification Potential in Seawater

This paper identifies a novel thermostable GH5 endoglucanase from Bacillus sp. strain BS that efficiently hydrolyzes cellulose and functions in seawater, reducing freshwater use in biofuel production.

Synthetic Yarrowia lipolytica consortium for efficient conversion of lignocellulosic oligosaccharides into lipids

This study demonstrates a synthetic Yarrowia lipolytica consortium that efficiently converts lignocellulosic oligosaccharides into lipids, with division-of-labor strategies outperforming multifunctional monocultures.

A Highly Conserved ABC Transporter Mediates Cello-Oligosaccharide Uptake in the Extremely Thermophilic, Lignocellulolytic Bacterium Anaerocellum (f. Caldicellulosiruptor) bescii

This study identifies an ABC transporter in Anaerocellum bescii responsible for cello-oligosaccharide uptake, showing genetic deletion impairs growth on cellulose and cellobiose.

Understanding the Dynamics of Biomass Deconstruction by the Cellulolytic Anaerobe C. thermocellum

This study uses advanced microscopy and clustering methods to analyze cellulosome distribution in C. thermocellum during growth on different substrates, supporting theories about their role in biomass deconstruction.

Plant Cell Wall Enzymatic Hydrolysis: Predicting Yield Dynamics from Autofluorescence and Morphological Temporal Changes

This study develops a pipeline to predict sugar conversion yields during plant cell wall enzymatic hydrolysis using autofluorescence and morphological changes, revealing strong negative correlations with conversion efficiency.

CAZyXplorer: A Shiny Application for Cost-Effective Preliminary Screening of Microbial Strains to Advance Enzyme Discovery in Biorefining and Biotechnology

CAZyXplorer is a Shiny application that screens microbial strains for enzyme potential in biorefining using CAZy annotations and multi-criteria decision analysis.

Identification of Diverse Cellulose Binding Domains using in silico Prioritisation and High-Throughput Screening

The paper develops a bioinformatics workflow to identify diverse cellulose-binding domains through in silico prioritisation and high-throughput screening, identifying 5 CBMs with significant binding to cellulose materials.

An evolved strain of Spathaspora passalidarum produces ethanol from sugarcane bagasse and switchgrass lignocellulosic hydrolysates

This study evolved Spathaspora passalidarum to produce ethanol from lignocellulosic hydrolysates by improving tolerance to inhibitors like acetic acid.

The Carbohydrate Binding Module of TrCel7A Aids in Navigating the Complexity of Plant Cell Walls

This study shows that the carbohydrate-binding module (CBM1) of TrCel7A improves enzyme binding and navigation on complex plant cell wall substrates.

Integrated Multi-analytical Framework for Comprehensive Characterization of Lignocellulosic Hydrolysates

This study develops an integrated multi-analytical workflow to comprehensively profile biomass hydrolysate for downstream valorization.

Domain Compatibility and Linker Design Dictate the Success of Chimeric Cellulase Engineering

This study designs chimeric cellulases by combining endoglucanases and β-glucosidases, finding that domain compatibility and linker design are critical for maintaining catalytic activity and stability.

Novel single-domain AA12 pyrroloquinoline quinone-dependent oxidoreductases directly and co-operatively drive lytic polysaccharide monooxygenase activity with AA8 module

This study identifies single-domain AA12 oxidoreductases and free AA8 modules as direct and cooperative electron donors to lytic polysaccharide monooxygenase (LPMO) in cellulolytic fungi.

Reprogrammed E. coli for secretion of thermophilic cellulase cocktail for seawater-compatible lignocellulosic bioprocessing

This paper engineers E. coli to secrete a thermophilic cellulase cocktail that functions in seawater, achieving high yields and activity for lignocellulosic bioprocessing.

Comparative evaluation on the fermentative potential of single and assorted fruit and vegetable waste for production of bioethanol

This study compares the fermentative potential of single and assorted fruit and vegetable waste for bioethanol production, finding that assorted waste has higher sugar content and ethanol yield.