Reproductive Biology and Fertility

This paper predicts oocyte competence in bovine using RNA-seq and WGBS from biopsies, cumulus cells, granulosa cells, and polar bodies, identifying transcriptional and epigenetic differences between cleaved but arrested and blastocyst-stage embryos.

This study shows that co-culturing immature human oocytes with hiPSC-derived ovarian support cells improves in vitro maturation rates and transcriptomic similarity to in vivo matured oocytes.

The paper identifies a macrophage-germline-somatic gonadal cell axis driven by BMP15 mutation that contributes to ovarian failure and masculization in zebrafish.

This study analyzes gene expression in granulosa cells of southern white rhinoceros to inform assisted reproductive technologies.

The paper investigates the role of GLP methyltransferase in oocyte development using multi-omics analysis and mouse models, finding that GLP contributes to transcriptional repression and is critical for oogenesis.

This study shows that KOMP Fshr heterozygous knockout mice do not exhibit accelerated ovarian aging phenotypes previously reported in another Fshr haploinsufficiency model.

This study introduces an electroporation method to suppress maternal effect genes in mouse oocytes without removing cumulus cells, showing efficient maternal mRNA suppression and revealing H3.3's role in post-fertilization development.

This study examines how altered hormonal environments affect pre-implantation mouse embryo movement in different diapause models, finding that both estrogen and progesterone influence embryo movement patterns.

This study examines how pyruvate metabolism affects histone acetylation and gene expression in bovine oocytes during in vitro maturation.

This study identifies CHK1's role in regulating cell cycle progression in early mouse embryos to maintain genome integrity and prevent aneuploidy.

This study shows that OVGP1, an oviductal humoral factor, is essential for female fertility and early embryonic development in golden hamsters.

The paper identifies a distinct transcriptional programme in pregranulosa cells before primordial follicle activation using single-cell RNA sequencing in mice.

This study evaluates two alternative culture systems for bovine oocyte in vitro maturation, finding no significant differences in meiotic resumption or embryo development compared to conventional 2D systems.

This study shows that maternal age and high fat diet reduce preimplantation embryo development in mice, with older age having a greater impact than diet.

The paper identifies Nestin+ perivascular cells in mice as multipotent progenitors contributing to ovarian lineages and shows their role in folliculogenesis through Notch signaling.

This study investigates the structural relationships between the ovary and surrounding tissues during mouse development, identifying key interactions that drive ovary morphogenesis.

This study uses t-BHP to induce ovarian follicle senescence in pigs, showing increased ROS, Caspase-3, P53, Foxo1, and decreased SOD, establishing an in vitro aging model.

This study shows that IVF-generated murine blastocysts exhibit increased Warburg metabolism and altered lactate production compared to naturally conceived embryos.

This study constructs a single-cell atlas of the murine ovary by analyzing >34,000 cells across the estrous cycle to characterize cell state dynamics and transcriptional changes.

The paper identifies lipid metabolism as a key factor in developmental pausing, showing that enhancing fatty acid oxidation extends embryo survival during mTOR inhibition.

This study shows maternal Nanog controls oocyte maturation and early embryonic development by repressing eef1a1l2 transcription, which reduces translational activity and prevents ER stress.

The paper shows that eIF4E1B regulates maternal mRNA translation to enable zygotic genome activation by protecting chromatin remodeling and reprogramming factor mRNAs from degradation.

This study shows that uterine deletion of Foxa2 in mice leads to embryonic diapause, with persistent Msx1 expression and altered estrogenic effects on uterine quiescence.

This study uses 3D analysis to show radial meiotic initiation and intercellular bridges in human fetal ovaries, with variation in meiotic cell numbers and asynchronous transitions between mitotic and meiotic stages.

The paper analyzes transcriptomes of arrested human embryos to identify molecular markers associated with blastulation failure and developmental stages.

The paper identifies nurse cells in mouse oocyte cysts that support oocyte development through cytoplasmic transfer and conserved mechanisms across species.

The paper develops a methodology for selecting IVF cases with oocyte maturation defects and preimplantation embryo lethality, using whole-exome sequencing and bioinformatics to identify causative genetic variants.

This study shows that reducing ooplasmic volume in oocytes improves meiotic accuracy and embryo development, enabling offspring production from azoospermic mice with spermatocyte arrest.

This study uses single-nuclei multi-omics analysis to identify molecular and cellular changes in human ovarian ageing and links genetic variants to age at natural menopause.

The paper uses machine learning to analyze embryo developmental timing and shows that maternally aged embryos lose temporal regulation, impacting viability.