Carbohydrate Chemistry and Synthesis

Chemoenzymatic Synthesis of 6-Sulfo Sialyl Lewisx Containing Glycans to Probe the Receptor Specificity of MERS Coronavirus

This paper synthesizes 6-sulfo sialyl Lewisx glycans to identify MERS-CoV's preferred receptor and develops a multivalent inhibitor.

Hexosyl transfer via a covalent intermediate within a dual retaining and inverting enzyme

This study reveals a covalent mechanism in a bifunctional glycosyltransferase for hexosyl transfer and reports a novel self-priming function for polymerization.

Structural mechanism defining product specificity in glycoside hydrolase family 66 cycloisomaltotetraose glucanotransferase

This study identifies a molecular mechanism in CI4Tase that enforces strict DP4 specificity for cycloisomaltotetraose synthesis through structural and mutational analysis.

Chemical Rescue Serves as a Predictive Proxy for Glycosynthase Activity on Glycosidic Bonds via a Shared Glycosyl Oxocarbenium Transition State

The paper demonstrates that chemical rescue is a predictive proxy for glycosynthase activity, validated through correlation with enzymatic activity and transition state analysis.

Rationally Engineered, Chemically Stable Tunicamycin Analogues Decouple DPAGT1 Inhibition from Non-Selective Toxicity

This paper develops chemically stable tunicamycin analogues that inhibit DPAGT1 selectively with reduced toxicity and improved pharmacokinetics.

Structural basis for recognition of a gonococcal lipooligosaccharide epitope by monoclonal antibody 2C7 informs vaccine and immunotherapeutic design

This study determines the structural basis for mAb 2C7 recognition of a gonococcal LOS epitope, identifying a minimal tetrasaccharide that retains key binding determinants for vaccine and therapeutic design.

GLYCAM Bacterial Carbohydrate Builder: a web-tool for modelling 3D structures of bacterial glycans

The paper introduces a web-tool for generating 3D structural models of bacterial glycans using the GLYCAM-Web Carbohydrate Builder.

One-pot enzymatic synthesis of the sugar nucleotide CDP-ribitol

This paper describes the one-pot enzymatic synthesis of CDP-ribitol from ribitol and explores its derivatives for glycoscience applications.

Tunable multivalent Fe(II)-based glycoassemblies as mimetics for native high-mannose glycans.

This study creates tunable Fe(II)-based glycoassemblies that mimic high-mannose glycans and inhibit their interactions with lectins.

Chemoenzymatically Synthesized O-Acetylated GD3 Gangliosides to Examine Viral Receptor Specificities in a Cellular Context

The paper describes a chemoenzymatic method to synthesize O-acetylated GD3 gangliosides and uses them to study viral receptor specificities in a cellular context.

C-6 Modified 2-F-Fucose Derivatives as Inhibitors of Fucosyl Transferases

The paper designs and evaluates C-6 modified GDP-2-F-Fuc analogs as FUT inhibitors, finding two compounds that potently inhibit FUT1, 3, 6, and 9 with minimal activity against FUT8.

Shaping the Glycan Landscape: Hidden relationships between linkage and ring distortions induced by carbohydrate-active enzymes

This study investigates how carbohydrate-active enzymes induce conformational changes in glycans through ring distortions, revealing mechanisms that influence enzyme specificity and inhibitor design.

Chemical Synthesis of Oligosaccharides Derived from Streptococcus pneumoniae Serotype 35B and D to Investigate Binding of Complement and Serum Factors

This study synthesizes oligosaccharides from Streptococcus pneumoniae serotypes 35B and 35D to investigate their binding to complement and serum factors, revealing the role of O-acetylation in immune evasion and vaccine efficacy.

Uncovering bacterial pseudaminylation with pan-specific antibody tools

This study develops pan-specific monoclonal antibodies to detect pseudaminic acids in bacteria, enabling deeper glycoproteomic analysis and improved phagocytosis of multidrug-resistant strains.

Chemoenzymatic Synthesis of Glycosphingolipids having an HNK-1 Epitope for Erythrocyte Cell Surface Remodeling

This paper develops a synthetic method to create sulfoglucuronyl glycosphingolipids with HNK-1 epitopes, demonstrating their role in complement-mediated erythrocyte lysis by anti-HNK-1 antibodies.

In vitro selection of cyclized, glycosylated peptide antigens that tightly bind HIV high mannose patch antibodies

The paper develops a method to select glycopeptides that bind HIV broadly neutralizing antibodies, achieving tight binding with KD as low as 0.5 nM.

Generating 3D Models of Carbohydrates with GLYCAM-Web

GLYCAM-Web provides interactive and API-driven carbohydrate 3D structure builders that assemble, resolve steric overlaps, and energy-minimize oligosaccharides up to 100 residues in under a minute.

Structural basis for the strict substrate specificity of beta-D-galactofuranosidase from Streptomyces sp. JHA19

The paper determines the crystal structure of a Galf-specific beta-D-galactofuranosidase from Streptomyces sp. JHA19, revealing the structural basis for its strict substrate specificity.

2,2-Di-Fluoro-Derivatives of Fucose Can Inhibit Cell Surface Fucosylation Without Causing Slow Transfer to Acceptors

This paper evaluates 2,2-di-fluoro-fucose derivatives as FUT inhibitors, finding they inhibit fucosylation without slow transfer to acceptors, and show feedback inhibition of GDP-Fuc biosynthesis.

Structure-function analysis of the cyclic beta-1,2-glucan synthase

The paper determines the structure of cyclic beta-1,2-glucan synthase from Agrobacterium tumefaciens and clarifies its mechanism of action.

A consensus-based and readable extension of Linear Code for Reaction Rules (LiCoRR)

This paper extends Linear Code to represent reaction rules in glycobiology, aiming to improve consistency and interoperability in glycan synthesis models.

DNA Encoded Glycan Libraries as a next-generation tool for the study of glycan-protein interactions

The paper introduces a DNA-encoded glycan library (DEGL-50) and demonstrates its use in studying glycan-protein interactions using PCR, qPCR, and NGS technologies.

Bacterial glycoengineering as a biosynthetic route to customized glycomolecules

This paper discusses bacterial glycoengineering as a method to produce customized glycomolecules using recombinant expression of carbohydrate biosynthesis pathways.