Listeria monocytogenes in Food Safety

No two are the same: Identification and characterization of novel hyperactive PrfA* variants in Listeria monocytogenes

The paper identifies and characterizes novel hyperactive PrfA* variants in Listeria monocytogenes, showing their diverse effects across multiple assays and emphasizing the need for comprehensive analysis.

Manganese scavenging is required for virulence of the cytosolic pathogen Listeria monocytogenes

This study shows that manganese scavenging via high-affinity transporters is essential for the virulence and intracellular survival of Listeria monocytogenes.

Cell recovery and DNA extraction methods skew relative abundance estimations of foodborne pathogens and spoilage organisms on stainless steel surfaces

Environmental Microbial Community Signatures Associated with Listeria spp. Detection in German Meat Processing Facilities

This study identifies microbial community signatures associated with Listeria spp. detection in German meat processing facilities, highlighting drains as key niches and linking specific taxa to Listeria presence.

Novel, highly divergent clones in Listeria monocytogenes serotype 4b in North America: Sublineages 782 and 1039, members of the hypervirulent clonal complex 2

The paper identifies two new sublineages of Listeria monocytogenes serotype 4b, SL782 and SL1039, found exclusively in North America, and describes their genetic and phenotypic characteristics.

On the role of cell chaining in the attenuation of a Listeria monocytogenes divIVA mutant

This study investigates how cell chaining in a Listeria monocytogenes divIVA mutant affects virulence, finding that it is not the morphology itself but PrfA- and SecA2-dependent processes that are primarily responsible for attenuation.

Neurotropic strains of Listeria monocytogenes preferentially invade enteric glial cells

This study shows neurotropic L. monocytogenes strains preferentially invade enteric glial cells in the gut, using actin-based motility to spread to neurons and potentially reach the brainstem.

Challenges of Dry Sanitization to Control Salmonella Dry Surface Biofilms

This study evaluates the effectiveness of various sanitizers against Salmonella dry surface biofilms formed under different hydration and drying protocols.

K-mer based method for finding sequence type-specific PCR primers for Listeria monocytogenes

This paper develops a k-mer-based method to design PCR primers for rapid, low-cost sequence typing of L. monocytogenes with 100% sensitivity and specificity.

Variable fluid mechanics explain why static efficacy tests overestimate sanitizer performance against Listeria

This study shows that static efficacy tests overestimate sanitizer performance against Listeria by not accounting for fluid dynamics and exposure differences in real-world applications.

Trehalose metabolism and its impact on PrfA activity in Listeria monocytogenes

This study investigates how trehalose metabolism affects PrfA activity in L. monocytogenes, identifying trehalose as a PTS-dependent sugar that supports growth without impacting PrfA.

Use of a plasmid containing a dual gene reporter system to assess the cell hydrophobicity of Listeria monocytogenes

This study uses a dual gene reporter plasmid to assess the hydrophobicity of Listeria monocytogenes strains by monitoring their attachment to solvents.

Understanding the Role of Pyruvate Dehydrogenase in Listeria monocytogenes Virulence

This study identifies PDH complex defects in L. monocytogenes as critical for intracellular survival, showing that PDH mutants fail to utilize PTS-dependent carbon sources, leading to reduced virulence.

Monoclonal antibody-based serotyping of Listeria monocytogenes provides new insights in epidemiology and virulence

This paper introduces a monoclonal antibody-based serotyping method for Listeria monocytogenes that improves discrimination of serovars, revealing SV1/2*, SV3, and SV7 in previously classified isolates.

Bacteroidales on Harvesters: Baseline Prevalence and Abundance

The paper assesses fecal contamination on harvester surfaces using qPCR and develops a portable LAMP assay for rapid hygiene assessment.

Listeria monocytogenes biofilm-derived cells show differential sigB expression on a food model and enhanced survival in simulated gastric conditions

This study shows that Listeria monocytogenes biofilm-derived cells have higher sigB expression and better survival in simulated gastric conditions compared to planktonic cells.

A metagenomic framework for rapid Listeria monocytogenes surveillance in food production environments

This paper develops a metagenomic framework for detecting Listeria monocytogenes in food environments, showing that direct metagenomics captures more strain diversity than culture-based methods.

Large-scale phenotyping and comparative genomics reveal genetic features of Listeria persistence in epithelial cells

This study identifies folate biosynthesis as critical for Listeria monocytogenes persistence in epithelial cells by regulating ActA levels and activity, revealing a metabolic pathway that enables vacuolar dormancy.

Amphitrophic Listeria monocytogenes causes one-third of invasive listeriosis yet remains undetected by clonal complex-based risk classification

The paper shows that a major fraction of invasive Listeria monocytogenes is misclassified by conventional clonal complex-based risk assessment and demonstrates that amphitrophic lineages, which combine virulence and persistence traits, constitute 31% of clinical isolates.

Amphitrophic Listeria monocytogenes: multi-dimensional genomic profiling reveals a third ecological strategy that challenges the virulence-persistence trade-off paradigm

This paper develops a multi-dimensional genomic scoring system to evaluate virulence, persistence, clonal context, and resistance in L. monocytogenes, revealing a third ecological strategy that challenges the virulence-persistence trade-off.

Lineage-specific macrophage programs dictate metabolic suppression and stress responses associated with VBNC-like states in Listeria monocytogenes

This study shows that lineage-specific macrophage environments shape Listeria monocytogenes' metabolic and stress responses, leading to different intracellular survival strategies.

Neutrophils are critical for placental and fetal infection with the human pathogen Listeria monocytogenes

The paper identifies maternal neutrophils as a survival niche for Listeria monocytogenes, facilitating placental and fetal infection during pregnancy.

Cross-Genera Amplification of prs/hlyA by Multiplex PCR Resulted in Misidentification of Enterococcus faecium as Listeria monocytogenes

The study found that a common PCR assay for Listeria monocytogenes misidentified Enterococcus faecium as L. monocytogenes due to primer cross-reactivity, highlighting a 66.7% false-positive rate in a Nigerian context.

The phage shock protein response of Listeria monocytogenes influences tolerance to the multipeptide bacteriocin garvicin KS

This study identifies the phage shock protein response as a key factor in Listeria monocytogenes susceptibility to the bacteriocin garvicin KS.

The microbiota of wooden cheese ripening boards is a rich source of antimicrobial-producing bacteria against Listeria monocytogenes

The paper identifies antimicrobial-producing bacteria in wooden cheese ripening boards that inhibit Listeria monocytogenes, with a focus on Bacillus safensis as a potential biocontrol agent.

Disentangling the impacts of abiotic and biotic environmental factors anddispersal dynamics on the pangenome fluidity of bacterial pathogens

This study investigates how environmental factors and dispersal dynamics influence pangenome fluidity in Listeria monocytogenes across three evolutionary lineages.

LAP Drives Monocyte-Mediated and Extracellular Translocation of Listeria monocytogenes Across the Placental Barrier

This study identifies two distinct pathways by which Listeria monocytogenes crosses the placental barrier, highlighting LAP's role in both monocyte-mediated and extracellular translocation.

Peracetic Acid Efficacy and Decay Kinetics in Poultry Processing under Chiller Conditions

This study models peracetic acid decay and Salmonella inactivation during poultry chilling, showing that PAA concentration and exposure time are critical for bacterial inactivation, especially in the presence of organic load.

Rapid and accurate quantification of viable Listeria monocytogenes with clonal specificity using microfluidic droplet digital PCR based technology

This study develops a microfluidic ddPCR method for rapid, accurate quantification of viable Listeria monocytogenes with clonal specificity, using CC-specific primers and PMA to distinguish viable from dead cells.

Three metabolic pathways replenishing the one-carbon pool collectively support growth and virulence of Listeria monocytogenes

This study identifies three metabolic pathways that replenish the one-carbon pool in Listeria monocytogenes, supporting its growth and virulence.