Biosensors and Analytical Detection

This paper develops and validates a smartphone-based lateral flow immunoassay for serum Cystatin-C, showing high accuracy and reliability in clinical samples.

This paper introduces BINOCULAR, a system for rapid, multiplexed detection of Mycoplasma using toehold switch sensors, achieving sub-5-minute readout times and 1-copy/µL sensitivity.

This study identifies Tween 20 as a surfactant that enhances colorimetric glucose sensing on paper by preserving enzyme activity during drying, improving detection limits and signal stability.

This paper develops a portable biosensor for rapid ASFV detection in whole blood using microfluidic paper-based devices, achieving high sensitivity and specificity without DNA extraction.

This paper develops a silicone ink for high-resolution hydrophobic barriers on paper, improving fluid control and diagnostic sensitivity in paper-based diagnostics.

The paper develops a machine learning model to improve LAMP assay design by identifying key thermodynamic features from primer sets, achieving high classification accuracy.

This paper develops a label-free diagnostic platform for HIV detection with smartphone integration, achieving attomolar sensitivity without sample pre-treatment.

This paper develops an AIoT-integrated system for automated AMR surveillance in food supply chains, achieving high accuracy and cost reduction.

This paper develops a portable smartphone-integrated RPA-CRISPR-Cas12a system for early detection of potato late blight using microneedle sampling, achieving high sensitivity and field usability.

The paper introduces a rapid, semi-quantitative LAMP-OSD test for detecting Enterococci in water, enabling visual readout and comparison with contamination standards.

This paper develops light-activated RAA systems using photocaged DNA polymerase or primers to enable precise, spatiotemporal control of nucleic acid amplification for field-ready diagnostics.

This paper develops a low-input, ultrasensitive diagnostic platform for detecting Avocado Sunblotch Viroid using RPA-CRISPR and nanopore-array fluorescence readout, achieving a detection limit of 1.68 copies/L.

This paper develops a CRISPR-based assay for rapid detection of cefixime resistance in Neisseria gonorrhoeae by analyzing penA gene mosaicism.

This paper optimizes a PCR-CRISPR-Cas12a assay for rapid detection of the blaOXA-1 gene, achieving results in under an hour with basic equipment.

ThermiQuant AquaStream is a portable device that enables real-time colorimetric LAMP assays in both microPADs and tubes with high sensitivity and precision.

This study develops a machine-learning strategy to improve error tolerance in image-based nanoparticle immunoassays for field-based diagnostics.

The paper introduces MobileLAMP, an open-source, low-cost device for isothermal nucleic acid amplification, demonstrating its effectiveness with colorimetric LAMP for RNA and DNA targets.

This paper presents a morphology-guided deep learning platform for nanoparticle agglomeration assays, achieving high sensitivity and recall for DNA and SARS-CoV-2 RNA detection.

This paper develops a CRISPR-Cas12a assay for rapid, sensitive, and specific detection of African Swine Fever Virus in diagnostic samples, achieving high sensitivity and specificity comparable to qPCR.

This paper introduces a flow-through solid-phase PCR platform for rapid, sensitive, and quantitative detection of multiple viral pathogens in a single reaction.

This paper develops a YOLO-based algorithm to automate LAMP result classification, achieving high accuracy and precision in detecting positive and negative cases.

This paper develops a low-cost, high-throughput multiplexed cytokine detection platform for TB diagnostics using amplified enzymatic silver metallization and cellphone imaging.

The paper develops a rapid, instrument-free, and specific nucleic acid detection method for Marek's Disease Virus (MDV-1) using CRISPR/Cas14a and RPA, achieving a detection limit of 24.6 copies/L and 100% accuracy in clinical samples.

This paper introduces a combo lateral flow assay (CLFA) that enables quantitative detection of analytes without external instruments, using AI-based image analysis for improved precision.

The paper develops qLAMP and RT-qLAMP assays for detecting five BRD-associated viruses with high sensitivity and specificity, and introduces a streamlined pipeline for assay development.

This paper develops a low-cost, equipment-free paper-plastic cassette for nucleic acid extraction, showing comparable results to commercial kits and suitable for point-of-care diagnostics.

The paper introduces LabCap, a low-cost, instrument-free platform for biomarker detection using hydrogel rings in glass capillaries, achieving sensitivity down to 0.1 ng/mL for CRP and NT-proBNP.

The paper introduces DISCO-LAMP, a novel LAMP assay for isothermal antigen detection, achieving high sensitivity and low background amplification.

The paper introduces a dual-sided heater-imager system for quantitative colorimetric isothermal nucleic acid amplification on paper, achieving temperature uniformity and real-time analysis.