Biochemical Analysis and Sensing Techniques

The paper identifies Bassoon as a marker for synapses between Type III cells and afferent nerve fibers in taste buds using immunocytochemistry and immunoelectron microscopy.

The paper identifies key residues in umami peptides and their binding to T1R1/T1R3 receptors using computational methods and proposes a decision rule for predicting umami peptides.

The paper examines neural connectivity in mouse taste buds, finding that most nerve fibers connect to a single cell type, but some branch to connect to multiple types, challenging the labeled-line hypothesis.

This study shows that early-life low-calorie sweetener consumption in rodents leads to long-term metabolic, behavioral, and neural impairments without weight gain.

This study uses fMRI to investigate taste representation in the human gustatory cortex and finds overlapping activation patterns for basic tastes.

The paper improves comparative modeling of mammalian bitter-taste receptors (TAS2Rs) by re-aligning sequences and constructing homology models, validated through site-directed mutagenesis and functional assays.

The paper shows that P2X2/P2X3 double-knockout mice develop strong fructose appetition, unlike TRPM5 KO mice, suggesting distinct mechanisms for fructose taste perception.

The paper shows that perturbing BLA to GC projections alters GC taste response dynamics, specifically reducing palatability-related activity in the late epoch without affecting taste identity information.

The paper reconstructs peripheral taste neuron morphologies and finds differential convergence in branching characteristics across neurons, suggesting functional differences based on input from taste-transducing cells.

The paper tests GAD65Cre as a reporter for Type I taste cells and finds it drives expression in multiple cell types, making it unreliable for specific function studies.

This study enhances GABAergic tone in the rNTS of rats to show it reconfigures neural activity related to taste and licking behavior, improving discrimination of palatable vs. unpalatable tastants.

This study finds that artificial saliva, commonly used as a neutral control in gustatory research, is perceived as aversive by participants, challenging its assumed neutrality.

This study uses calcium imaging to show that taste representations in the gustatory insular cortex of awake mice are sparse and distributed, lacking spatial clustering or topography.

The paper identifies a new subset of Type III taste cells that respond to multiple taste stimuli and uses genetic and imaging approaches to characterize their signaling pathways.

The paper investigates genetic factors influencing dietary fat perception using twin studies and GWAS, finding genetic variants associated with potato chip liking.

This study models the human tongue as a porous medium to understand taste perception timing, finding that molecular size affects the delay and intensity of taste perception.

This study investigates how the gustatory cortex of mice encodes chemosensory and hedonic features of taste during active licking, showing rapid encoding of taste identity and licking-related activity.

The paper performs single-cell RNA sequencing on geniculate ganglion neurons to analyze taste modality-specific clusters and compares results with prior studies.

This study investigates whether diet or weight gain causes taste deficits in diet-induced obese mice, finding that both factors contribute to reduced taste responses.

This study uses high-resolution fMRI to show that taste quality is represented by a combinatorial spatial code rather than a topographic map in the human brain.

The paper uses serial blockface scanning electron microscopy to describe the morphology and distribution of cell types in mouse circumvallate taste buds.

The paper uses fMRI and multivariate pattern analysis to show that taste maps in human gustatory cortex change with stimulus concentration, revealing distinct spatial layouts for different taste categories at varying concentrations.

Maternal consumption of non-nutritive sweeteners during pregnancy increases adiposity and insulin resistance in mouse and human offspring.

The paper records spiking activity in mouse gustatory cortex during taste stimulation, finding temporal coding of taste identity and palatability and no anatomical taste specialization.

This study uses molecular dynamics simulations to investigate how different umami ligands affect the structural dynamics of the T1R1-T1R3 taste receptor, identifying key residues and binding sites involved in ligand recognition.

This study examines how obesity affects taste response patterns in the nucleus of the solitary tract in rats, finding reduced but more frequent responses in obese rats.

The paper investigates how bitter taste and nociceptive stimuli are linked in mouse parabrachial nucleus neurons using electrophysiology and optogenetics.

The paper investigates responses of mouse trigeminal ganglion neurons to kokumi substances using in vivo confocal Ca2+ imaging, finding that these substances evoke responses in a small fraction of neurons.

The paper investigates how sucrose intensity is encoded and used for decision-making in rat gustatory cortices, finding that a few intensity-selective neurons convey most of the information.

The paper investigates whether TAS2R38 mRNA expression in taste tissue correlates with expression in sinonasal tissue among heterozygotes, finding no significant relationship.