Metalloenzymes and iron-sulfur proteins

The paper identifies a C-terminal tripeptide that directs Fe-S cluster insertion into cytosolic and nuclear proteins by binding to the CIA-targeting complex.

This study investigates the role of a Cys pair in the DNA binding domain of Gd-NifA, finding it does not regulate DNA binding or dimerization through redox changes.

The paper shows that reducing trace metal and electron resources to nitrogenase allows engineered Azotobacter vinelandii to recover wild-type growth with a reduced nitrogenase variant.

The paper develops a logical model of Fe-S cluster biogenesis in E. coli to analyze how environmental stressors like iron deprivation and oxidative stress regulate this process.

The paper investigates why iron-sulphur cluster enzymes are often inactive when heterologously expressed in E. coli and identifies strategies to improve their functionality.

The paper shows that reducing histone H3-mediated Cu1+ production restores Fe-S cluster deficiencies in yeast, linking chromatin and mitochondrial function to Fe-S cluster homeostasis.

The paper analyzes the transcriptional regulation of nitrogen fixation in Paenibacillus durus under nitrogen-enriched and deficient conditions, finding that nifH transcripts are present but nitrogen fixation activity is absent, suggesting post-transcriptional or post-translational regulation.

This study uses homologous recombination to create ferredoxin chimeras, revealing how thermostability and midpoint potential are inherited differently.

The paper shows that protein-bound molybdenum cofactor (Moco) is bioavailable and rescues Moco-deficient C. elegans, suggesting a new therapeutic strategy for human Moco deficiency.

This paper designs chemical-dependent protein switches to control intracellular electron transfer by integrating ligand-binding domains with ferredoxin proteins.

This paper engineers nitrogen fixation activity in a non-diazotrophic cyanobacterium by transferring nitrogenase genes and improving O2 tolerance, achieving over 30% N2 fixation activity.