Viral Infectious Diseases and Gene Expression in Insects

The paper introduces a modular cloning toolkit for constructing multigene vectors compatible with both baculovirus and yeast expression systems.

This study develops and validates a fully animal component-free media for adherent mammalian cell lines, showing comparable morphology, growth, and transcriptomic profiles to FBS-based media.

The paper knocks out Bcl-xL in MDCK cells to reduce tumorigenicity, creating a safer cell line for influenza vaccine production.

This study identifies cellular adaptations in CHO cells linked to antibody production, finding that transgene copy number and mRNA expression levels are key factors in productivity.

The paper identifies stress-responsive biomarkers in CHO cells that could be used to engineer stress-resistant cell lines for improved bioreactor productivity.

The paper introduces a software toolkit for simulating viral transduction and propagation in biomanufacturing to optimize genetic and process designs.

This paper develops residual hybrid models combining mechanistic and machine learning to improve predictions of N-glycosylation and biopharmaceutical production, achieving significantly lower prediction errors.

This study examines how bioreactor pH affects CHO cell metabolism and N-linked glycosylation of IgG antibodies using flux balance analysis and glycopeptide mapping.

The paper compares transcriptomic changes in CHO cells producing different recombinant mAbs, finding adaptive mRNA abundance changes linked to mAb production and cellular capacity.

This paper introduces an episomal plasmid system for stable production of recombinant proteins and engineered antibodies in Expi293 cells.

This paper develops a non-invasive plate reader assay for high-throughput mammalian cell analysis, showing its applicability to different cell lines and conditions.

The paper uses AI and machine learning to identify key targets in signaling pathways related to CHO cell death, aiming to optimize biopharmaceutical production.

The paper develops a mechanistic model of rAAV production in BEVS, identifying Rep78 levels and transgene expression as key factors limiting yields.

The paper identifies a novel chimeric RNA formed between silkworm HDAC11 and BmCPV S4 transcripts that inhibits viral proliferation by altering histone modifications.

The paper identifies transcriptomic signatures that explain most of the variation in recombinant protein yield in CHO cells producing 2165 human secretome proteins.

The paper compares transcriptomic profiles of HEK293 cell lines producing erythropoietin or GFP to identify factors associated with improved erythropoietin production.

The paper identifies secretory pathway differences between HEK293 and CHO cells that improve recombinant protein expression when switching hosts.

The paper optimizes transfection and culture parameters in HEK293E cells to enhance recombinant antibody production, achieving a 4-fold increase in light chain production and a 2.5-fold increase in whole antibody production.

The paper generates and tests short artificial introns to enhance transgene expression in mammalian cells, identifying 12 effective variants.

The paper describes an in-vitro glycoengineering approach to modify N-glycans on recombinant SARS-CoV-2 spike glycoprotein to mimic mammalian cell-derived glycoforms.

The paper demonstrates the use of CRISPR/Cas9 and PIG bombardment to edit the genome of Trichoplusia ni granulovirus in vivo, achieving reporter protein expression in infected larvae.

This study analyzes transcriptome changes in silkworm prothoracic glands after BmNPV infection, identifying genes potentially involved in precocious metamorphosis.

The paper presents genome assemblies and gene predictions for Sf21 and Tni cell lines using multi-omics data, enabling improved use in protein expression and genome engineering.

The study constructs a shuttle vector with CMV and OpIE2 promoters to express genes in mammalian and insect cells, finding that OpIE2 promoter sequences inhibit CMV promoter activity in mammalian cells.

The paper describes modified E. coli strains that block native Tn7 attachment sites to reduce contamination from non-recombinant baculovirus production.

CHOmics is a web-based tool for analyzing and visualizing multi-omics data from CHO cell lines, offering integrated analysis, customization, and visualization features.

The paper analyzes genomic, transcriptomic, and metabolic changes in HEK293 cell lines to understand genetic and compositional differences between adherent and suspension derivatives.

This study uses a genome-scale metabolic network model to design feeding strategies for CHO cells, resulting in a two-fold increase in monoclonal antibody production.

This study investigates the effects of elevated ammonia on genome instability in CHO cells, identifying de novo mutations and microsatellite instability as biomarkers for genome stability.