Advanced NMR Techniques and Applications

The paper presents a workflow combining CCPNMR2.4 and ARIA2.3 to handle N-terminal acetylation and C-terminal amidation in protein structure calculation, showing no significant difference from existing methods.

Interact automates analysis of 1D and 2D NMR titration experiments to quantify protein-ligand interactions through peak picking, annotation, and fitting procedures.

The paper describes the synthesis and purification of 5,5,5-trifluoroisoleucine and its perdeutero and 5-13C variants, along with 2-13C-trifluoroacetate, for use as precursors in introducing the 13CF3 group into proteins.