Barrier Structure and Function Studies

The paper develops a human neurovascular culture model to study TBI effects, finding incomplete vascular repair and glial-mediated signaling changes.

This study shows that deleting Hdac2 in adult CNS endothelial cells reactivates pro-angiogenic programs, promoting revascularization and neuronal regeneration after stroke.

This study identifies metabolic and lipidomic changes in mouse brain endothelial cells undergoing senescence in vitro, highlighting alterations in tight-junction proteins and key metabolic pathways.

This study investigates how NS1 proteins from high- and low-pathogenicity TBEV strains interact with BBB tight junction proteins, revealing strain-specific binding patterns that may explain differential neuropathogenicity.

This study shows that Plasmodium falciparum egress products disrupt BBB endothelial junctions and activate JAK-STAT signaling, leading to increased permeability and suggesting therapeutic targets.

The paper investigates how Angubindin-1 disrupts the blood-tumor barrier by targeting Angulin-1/LSR, enhancing drug permeability and reducing glioma progression.

This study uses a novel preparation to visualize and quantify cardiac pericytes under physiological conditions, revealing their extensive organization and potential roles in signaling and contractile support.

This study shows that aging increases pulmonary microvascular endothelial cell permeability due to impaired cell-cell junctions and actin cytoskeleton changes, not inflammation.

This study develops humanized TfR1 and Tf gene-replacement rats to evaluate BBB transport of human-specific therapeutics, showing viability and partial functional compensation.

This study identifies the Rho-ROCK pathway's role in cleaving EpCAM/TROP2 to release claudin for rapid de novo tight junction formation during epithelial turnover.

This paper demonstrates that dual targeting of TfR and CD98hc enhances brain exposure of large molecules compared to single-target approaches.

This study shows that myeloid cell-driven transdifferentiation of endothelial cells into pericytes helps restore BBB function and brain repair after stroke.

This study identifies isoDGR-induced endothelial cytoskeletal disruption as a key driver of age-related BBB breakdown and shows that targeting isoDGR preserves BBB integrity and reduces neuroinflammation.

3BTRON is a deep learning framework that automatically analyzes electron microscopy images to distinguish BBB structures in aged versus young mouse brains, achieving 77.8% sensitivity and 80.0% specificity.

This study shows that Group B Streptococcus (GBS) causes meningitis by lysing endothelial cells in zebrafish, not through transcytosis, and identifies inflammation and clotting as key factors in brain invasion.

This study identifies vascular luminal glycocalyx loss and complement activation as key drivers of BBB injury in hyperglycemic stroke, showing that targeting complement after reperfusion improves outcomes.

This study shows that blocking VCAM1 or VLA4 after stroke preserves vascular integrity and prevents cognitive decline by promoting blood vessel growth and restoring the blood-brain barrier.

This study examines how brain precapillary sphincters regulate capillary blood flow in adult and aged mice during blood pressure surges, showing diminished myogenic response in older mice.

The paper develops a synthetic antibody fragment that binds to 10 claudin subtypes, opening tight junctions and modulating paracellular permeability.

The paper investigates how high-density lipoproteins (HDL) cross the blood-brain barrier, showing that LDLR and apoE composition determine HDL entry and brain localization.

The paper shows that 25-hydroxycholesterol disrupts brain endothelial function by altering cholesterol metabolism, linking viral infection to brain vessel dysfunction in intracerebral haemorrhage.

This study shows that Epstein-Barr virus and Helicobacter pylori coinfection disrupts gut and brain barriers by altering junction proteins and inducing neuroinflammation.

This study shows that acute high-fat high-sugar diet increases blood-brain barrier permeability in mice within one hour.

This study identifies distinct Ca2+ signaling profiles in leptomeningeal fibroblast subgroups in awake mice under health and inflammation conditions.

This study investigates how murine cerebral capillary endothelial and pericyte cells respond to blood flow cessation, finding that pericytes mediate microvascular constriction via ET-1 and alter endothelial tight junctions.

This paper optimizes a protocol for isolating murine BBB capillaries and evaluates its effectiveness in reducing non-vascular and non-capillary material.

The paper presents techniques to quantify the horizontal and vertical distribution of junctional proteins in fixed epithelial cells under different experimental conditions.

This study identifies vessel-type-specific vascular barrier dysfunction in Huntington's disease and shows partial rescue with an S1PR1 agonist.

This paper develops humanized TfR1-targeting nanobodies (NewroBus) that cross the BBB and deliver therapeutic payloads to the CNS.

This paper develops a 3D microfluidic chip model of the neurovascular unit that supports neural-vascular interactions and is used to study neuroinflammation and drug transcytosis.