Synthetic BiologybioRxivPreprint
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Integrating Genetic Domestication and Model-Guided Kill-Switch Engineering in a Non-Model Bacterial Therapeutic
Yeonhwee Jeong, Hari R. Namboothiri, Oluwatola Femi-Olatunji…·Chelsea Y. Hu
Host-measured parts and inducible systems for Brucella melitensis ΔvjbR guided a modeled Cas9 kill-switch, which cut mouse liver bacterial burden by about 91% at 30 mg/kg doxycycline.
DOI 10.64898/2026.10.18.759933